We narrowed to 29,617 results for: PLE

1. T2m

   Plasmid

   #121029

   Purpose

   MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   ECK120033736 (164.6x Voigt terminator), short attachment
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. T7m

   Plasmid

   #121030

   Purpose

   MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   S. pyogenes tracrRNA terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. T13m

   Plasmid

   #121032

   Purpose

   MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ECK120033737 (second strongest natural Voigt terminator)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C100m

   Plasmid

   #121004

   Purpose

   MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   sfYFP with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C41m

   Plasmid

   #120974

   Purpose

   MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. C61m

   Plasmid

   #120977

   Purpose

   MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   RpaR from Scott and Hasty 2016
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C62m

   Plasmid

   #120978

   Purpose

   MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   TraR(W) from Scott and Hasty 2016
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. P12m

   Plasmid

   #120938

   Purpose

   MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ohAB carb pLasTetO
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. P15m

   Plasmid

   #120939

   Purpose

   MoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   ohAB carb pCinTetO
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P16m

    Plasmid

    #120940

    Purpose

    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ohAB carb pCinLacO m
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. P32m

    Plasmid

    #120943

    Purpose

    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    PlacO (Tim Lu, Matt Bennett, etc)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P33m

    Plasmid

    #120944

    Purpose

    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    P11 from Mutalik library (medium)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. P34m

    Plasmid

    #120945

    Purpose

    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    P13 from Mutalik library (weak)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCW57.1

    Plasmid

    #41393

    Purpose

    3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBScrispri Sham

    Plasmid

    #223201

    Purpose

    Group B Streptococcus inducible dCas12 CRISPRi with Modular crRNA

    Depositor

    Thomas Hooven

    Insert

    N/A
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid

    #72259

    Purpose

    Express FLPe and tdTomato under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T230I in tdTomato (please see depositor's co…

    Promoter

    CB promoter and chicken beta-actin + CMV enhancer

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSG5-Crkl mut SH3

    Plasmid

    #41705

    Depositor

    Nora Heisterkamp

    Insert

    Crkl (CRKL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W160K

    Promoter

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

18. C39m

    Plasmid

    #120972

    Purpose

    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ccdB toxin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site removed

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAd:TRE-gateway

    Plasmid

    #30217

    Depositor

    William Pu

    Article

    He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

20. HA-tagged Abr

    Plasmid

    #38190

    Depositor

    Nora Heisterkamp

    Insert

    ABR (ABR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only