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We narrowed to 24,316 results for: crispr

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Showing: 5121 - 5140 of 24316 results

  1. pZNF70-donor

    Plasmid
    #112318
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF70 with GFP
    Depositor
    Kevin White
    Insert
    ZNF70 homology arms flanking EGFP-IRES-Neo cassette (ZNF70 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112318-plasmid-map-sequence-id-228523

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  2. pZNF266-donor

    Plasmid
    #112306
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF266 with GFP
    Depositor
    Kevin White
    Insert
    ZNF266 homology arms flanking EGFP-IRES-Neo cassette (ZNF266 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112306-plasmid-map-sequence-id-228458

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  3. pZSCAN32-donor

    Plasmid
    #112304
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF434 with GFP
    Depositor
    Kevin White
    Insert
    ZNF434 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN32 Human)
    Use
    CRISPR
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112304-plasmid-map-sequence-id-228421

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  4. pZNF165-donor

    Plasmid
    #112302
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF165 with GFP
    Depositor
    Kevin White
    Insert
    ZNF165 homology arms flanking EGFP-IRES-Neo cassette (ZNF165 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112302-plasmid-map-sequence-id-228414

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  7. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  13. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  15. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid
    #139999
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139999-plasmid-map-sequence-id-346119

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  18. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid
    #139994
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139994-plasmid-map-sequence-id-275519

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Showing: 5121 - 5140 of 24316 results