Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 30,690 results for: des.2

Clear keywords
Showing: 5121 - 5140 of 30690 results

  3. pGTM_Sumo2-C-8XHis-PLpro

    Plasmid
    #234321
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage an 8xHis Tag is retained at the C-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234321-plasmid-map-sequence-id-469248

    Enlarge

  4. pGTM_Sumo2-N-StrepII-PLpro

    Plasmid
    #234322
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage a Strep-tag II sequence is retained at the N-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and Strep-tag II
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234322-plasmid-map-sequence-id-469910

    Enlarge

  5. pGTM_Sumo2-N-8XHis-Plpro-C111S

    Plasmid
    #234472
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 C111S with an activity abolishing mutation for the purification of this PLpro protease. Following Sumo Protease Cleavage an N-term 8xHis Tag is retained
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234472-plasmid-map-sequence-id-469247

    Enlarge

  6. pGTM_Sumo_PLpro_C111A

    Plasmid
    #234489
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a C111A active site mutation that abolishes protease activity. Following Sumo Protease cleavage no nonnative res
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    234489-plasmid-map-sequence-id-469250

    Enlarge

  7. pGTM_SUMO_PLpro_C111S

    Plasmid
    #233740
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a C111S active site mutation that abolishes protease activity. Following Sumo Protease cleavage no nonnative res
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    PLpro (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    Cys111Ser active site mutation of the Papain-Like…
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    233740-plasmid-map-sequence-id-469323

    Enlarge

  8. pGTM_Sumo2-N-8XHis-Plpro

    Plasmid
    #233803
    Purpose
    Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of an active PLpro protease. Following Sumo Protease Cleavage an 8xHis Tag is retained at the N-terminus
    Depositor
    Gaetano Montelione
    Article
    De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do
    Insert
    orf1ab (ORF1ab SARS-CoV-2)
    Tags
    6xHis-Sumo and 8xHis
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    Feb. 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    233803-plasmid-map-sequence-id-469494

    Enlarge

  9. pCAGGS-S-P1-FLAG

    Plasmid
    #177095
    Purpose
    P1 variant S-protein with c-terminal FLAG tag
    Depositor
    Daniel Conway
    Insert
    S-protein P1 (S SARS-CoV-2)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Oct. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177095-plasmid-map-sequence-id-345528

    Enlarge

  10. pMCSG53_Nsp3_Unique

    Plasmid
    #167258
    Purpose
    Bacterial expression of Nsp3 Macrodomain 2 and Macrodomain 3 (residues 413-676)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 413-676) (ORF1ab SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available Since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167258-plasmid-map-sequence-id-331340

    Enlarge

  11. pCAGGS-S-501V2-FLAG

    Plasmid
    #177096
    Purpose
    501V2 variant S-protein with c-terminal FLAG tag
    Depositor
    Daniel Conway
    Insert
    S-protein 501V2 (S SARS-CoV-2)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Nov. 2, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177096-plasmid-map-sequence-id-346793

    Enlarge

  12. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available Since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

    Enlarge

  13. pAAV2_Rep52-VP1

    Plasmid
    #232173
    Purpose
    Expression of replication/capsid proteins for AAV serotype 2
    Depositor
    Michael Wehr
    Insert
    Rep52, VP1 of AAV-2
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    232173-plasmid-map-sequence-id-466763

    Enlarge

  14. pcDNA216-CoV2-RBD-gamma variant

    Plasmid
    #194600
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 gamma
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-gamma variant (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194600-plasmid-map-sequence-id-381528

    Enlarge

  15. pcDNA216-CoV2-RBD-delta variant

    Plasmid
    #194601
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 delta
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-delta variant (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194601-plasmid-map-sequence-id-381523

    Enlarge

  16. pcDNA216-CoV2-RBD-alpha variant

    Plasmid
    #194599
    Purpose
    Mammalian expression of RBD domain from SARS-CoV-2 alpha
    Depositor
    Ben Murrell
    Insert
    CoV2-RBD-alpha variant (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Available Since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    194599-plasmid-map-sequence-id-381522

    Enlarge

Showing: 5121 - 5140 of 30690 results