We narrowed to 13,665 results for: crispr cas9

1. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA1_GAL4UAS-Luciferase reporter

   Plasmid

   #64157

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMG

   Plasmid

   #138264

   Purpose

   Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Inserts

   eGFP flanked by iCas9 sites
   mCherry
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Tef1

   Available Since

   March 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA2_GAL4UAS-Luciferase reporter

   Plasmid

   #64158

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIK198

   Plasmid

   #65629

   Purpose

   "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Promoter

   U6

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-EAMP

   Plasmid

   #138265

   Purpose

   mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   iCas9 target-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a-HTLV

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPEY-Z3-Kan

   Plasmid

   #232102

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPEY-Z3-Hyg

   Plasmid

   #232101

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-hsg(NT:NT)

   Plasmid

   #138260

   Purpose

   Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   Nontargeting sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB1C3-FeGFP

    Plasmid

    #138261

    Purpose

    Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    eGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    None

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS280

    Plasmid

    #73738

    Purpose

    Simple cloning vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTHAP12-N1.1.2-gDNA

    Plasmid

    #175860

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    THAP12-Nickase2
    

    Use

    CRISPR

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCHCHD3-N2.1.2-gDNA

    Plasmid

    #175858

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    CHCHD3-Nickase2
    

    Use

    CRISPR

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCHCHD3-N1.1.1-gDNA

    Plasmid

    #175857

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White, Barbara Stranger

    Insert

    CHCHD3-Nickase1
    

    Use

    CRISPR

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZNF668.1.0-gDNA

    Plasmid

    #132463

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF668 (ZNF668 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCENPBD1.1.0-gDNA

    Plasmid

    #132460

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    CENPBD1 (CENPBD1 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTHAP7.1.0-gDNA

    Plasmid

    #132439

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    THAP7 (THAP7 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJKW0474

    Plasmid

    #107588

    Purpose

    pCLV3:Cas9

    Depositor

    Jing-Ke Weng

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Arabidopsis CLAVATA3

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458_ZNF219_1

    Plasmid

    #72368

    Purpose

    Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ZNF219 (ZNF219 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458_ZNF219_2

    Plasmid

    #72369

    Purpose

    Encodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Insert

    ZNF219 (ZNF219 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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