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pHybr_r2a>mG1-srt-his

Plasmid

#124802

Purpose

HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.

Depositor

Ferenc Scheeren, Martijn Verdoes

Publication

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

Murine IgG1

Use

CRISPR; Hdr template

Tags

Histag (HHHHHH) and Sortag (LPETGG)

Expression

Bacterial and Mammalian

Promoter

-

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHBS1413 GFP-TDP43 VLIM-F

Plasmid

#118802

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Publication

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All VLIM to F in CTD of full-length TDP43

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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aL-mEos3.2

Plasmid

#113154

Purpose

constrained LFA-1-mEos3.2 fusion

Depositor

Timothy Springer

Publication

Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

Insert

full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)

Tags

mEOS3.2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4b (mEosFP4b-V69T)

Plasmid

#99226

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

Depositor

Periklis Pantazis

Publication

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4b

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5C6A,P-J23106-geneIII

Plasmid

#80860

Purpose

Phagemid (encodes evolved cI5C6A,P and M13 gene III)

Depositor

Mark Isalan

Publication

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mPapaya1-H2B-6

Plasmid

#56651

Purpose

Localization: Nucleus/Histones, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

H2B (H2BC11 Human)

Tags

mPapaya1

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_CS M_tTA-BFP

Plasmid

#58875

Purpose

MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Atg7_L (OZ557)

Plasmid

#28082

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Atg7 (atg7 Zebrafish)

Use

Zebrafish targeting

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
Atg7_R (OZ558)

Plasmid

#28083

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Atg7 (atg7 Zebrafish)

Use

Zebrafish targeting

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pSuperRetro mouse Hic-5

Plasmid

#11187

Depositor

Bruce Spiegelman

Publication

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (Tgfb1i1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pSB-XPRESSO-hSpCas9-EGFP

Plasmid

#237298

Purpose

Sleeping Beauty XPRESSO vector for Cas9-EGFP expression

Depositor

Lior Gepstein

Publication

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

spCas9 (cas9 Stretococcus pyogenes)

Use

CRISPR

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4 human alpha4 full

Plasmid

#221397

Purpose

Mammalian expression of human integrin alpha4 full length

Depositor

Timothy Springer

Publication

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha4 full length (ITGA4 Human)

Tags

Strep twin

Expression

Mammalian

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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All-in-one split ADAR2(E488Q)

Plasmid

#170137

Purpose

AAV vector carrying ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Publication

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC(E488Q)-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAG671 mito-pFAST

Plasmid

#172867

Purpose

Expresses mitochondrial targeting domain-pFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

mito-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Publication

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

TEF1p

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-CB5

Plasmid

#171007

Purpose

HiLITR protease with CB5 C-terminal tmd targeting information (ER)

Depositor

Alice Ting

Publication

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-CB5(tmd)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-G418

Plasmid

#160215

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-G418

Plasmid

#160023

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-G418

Plasmid

#160018

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgH (TB40/E)

Plasmid

#136446

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain), full-length

Depositor

Erik Procko

Publication

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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