Addgene

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pME-cas9-2A-mCherry

Plasmid

#107592

Purpose

An entry vector with cas9 -2A-mCherry

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

cas9-2A-mCherry

Use

CRISPR

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-gRNA-EF1a-EGFP-CAAX

Plasmid

#167936

Purpose

gRNA entry vector

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

U6-gRNA backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBA560

Plasmid

#186236

Purpose

pTet-eLbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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BRAF in pENTR

Plasmid

#216605

Purpose

gateway cloning donor vector containing BRAF

Depositor

Douglas Allan, Christopher Loewen

Insert

BRAF (BRAF Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223 NRF2

Plasmid

#118708

Purpose

entry clone for NRF2

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

NRF2

Use

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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CTNNB1 in pENTR

Plasmid

#216617

Purpose

gateway cloning donor vector containing CTNNB1

Depositor

Douglas Allan, Christopher Loewen

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265_OoA3GX2

Plasmid

#225147

Purpose

Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

OoA3GX2-zCas9(D10A)-UGI

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pV1093

Plasmid

#111428

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pR562

Plasmid

#207886

Purpose

Creation of Level 0 entry clones

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

amilCP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK094-LM-AMA002.0

Plasmid

#171366

Purpose

Entry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLP1

Plasmid

#209988

Purpose

Entry vector to store Level 0 parts

Depositor

Thorsten Mascher, Georg Fritz

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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JD553

Plasmid

#160392

Purpose

Wheat GRF4-GIF1 chimera in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

wheat GRF4-GIF1 chimera

Use

Entry clon

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pME-ERT2-Cre-ERT2

Plasmid

#82587

Purpose

Middle entry vector containing tamoxifen-inducible estrogen receptor (ERT2) fused to both ends of CRE recombinase for tamoxifen-inducible Cre recombination

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

ERT2-CRE-ERT2

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Expression

Mutation

NLS fusion

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGEP179_pX330K

Plasmid

#137882

Purpose

Entry vector for multi-gRNA cloning

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pBA562

Plasmid

#186237

Purpose

pTet-RfxCas13d crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pME-secAnxaV-NS

Plasmid

#67718

Purpose

Gateway entry clone containing secreted human AnxaV with no stop codon

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

secAnxaV-NS

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMHG-W57* reporter

Plasmid

#72850

Purpose

GFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.

Depositor

Daniel Liebler

Article

Halvey et al FEBS Open Bio. 2012 Dec;2:56-59. Epub 20

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR hH1-TERRA

Plasmid

#111381

Purpose

TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences under control of hH1 promoter and terminator

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

hH1-TERRA

Use

Gateway entry cloning

Tags

Expression

Mutation

Promoter

hH1

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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