We narrowed to 43,816 results for: Sele
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Plasmid#160741PurposeExpresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobodyDepositorInsertHA-nGFP-(EAAAK)4-OGT(4,K852A)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-R244Q
Plasmid#109023Purposestable lentiviral expression of cDNADepositorInsertATL2-R244Q (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationArg244Gln mutation results in dimerization defectPromoterEIF-1a promoterAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
JP705: pMVP/SB/Neo-DEST
Plasmid#121866PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…ExpressionMammalianAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HOXC4 (human) MSCV
Plasmid#8546DepositorInsertHOXC4 (HOXC4 Human)
UseRetroviralExpressionMammalianMutationContains improved ribosomal binding site and NcoI…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#178107PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick exon-53 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA
Plasmid#178110PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G5
Plasmid#86618PurposeExpresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1DepositorInsertATP1A1 G5 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via hdr using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330_ATP1A1_G3_Dual_sgRNA
Plasmid#173205PurposeCoselection for HDR in human cells. Vector for dual expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.DepositorInsertATP1A1 G3 sgRNA + user-specified sgRNA + SpCas9
UseCRISPRExpressionMammalianPromoterDual U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA
Plasmid#173214PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
UseCRISPR; Prime editing (pe3)ExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA Flag Yap1 Y357E
Plasmid#18883DepositorAvailable SinceAug. 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB419
Plasmid#68684PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB420
Plasmid#68685PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB422
Plasmid#68687PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA
Plasmid#178096PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + MTOR nick exon-44 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA
Plasmid#178099PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + MTOR nick intron-45 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA
Plasmid#178102PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA
Plasmid#178103PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA
Plasmid#178105PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick exon-44 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA
Plasmid#178106PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G8 + MTOR nick intron-45 sgRNAs
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA
Plasmid#178108PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA
Plasmid#178109PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA
Plasmid#173209PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA
Plasmid#173211PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
UseCRISPR; Prime editing (pe3)ExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA
Plasmid#173212PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
UseCRISPR; Prime editingExpressionMammalianPromoterTandem U6 promotersAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G3
Plasmid#86617PurposeExpresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1DepositorInsertATP1A1 G3 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB418
Plasmid#68683PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB421
Plasmid#68686PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCTL-mlpx-neo
Plasmid#65233Purposeencodes a non-targeting control shRNADepositorInsertnon-coding shRNA
ExpressionMammalianPromoterPGKAvailable SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R53A)-Venus
Plasmid#138197PurposeFRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)
TagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]
Plasmid#138198PurposeFRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Mouse, Synthetic)
TagsCerulean and cpV[E172]ExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R466A)-Venus
Plasmid#138199PurposeFRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
TagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(L426W)-Venus
Plasmid#138200PurposeFRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
TagsCerulean and VenusExpressionMammalianMutationContains leucine-to-tryptophan substitution at re…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
ExpressionBacterialMutationA309GAvailable SinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Parkin
Plasmid#23956PurposeMammalian expression of human Park2 fused to mCherryDepositorAvailable SinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 gamma
Plasmid#20353DepositorAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nv5-ER beta
Plasmid#22770DepositorAvailable SinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-FAM134B-LIRmut
Plasmid#109027Purposestable lentiviral expression of cDNADepositorInsertFAM134B-LIRmut (RETREG1 Human)
UseLentiviralTagseGFPExpressionMammalianMutationDDFELL to DDAEAL aa 453-458 to interrupt FAM134B …PromoterEIF-1a promoterAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-ExRai-AktAR2
Plasmid#184050PurposePlasma membrane-targeted ExRai-AktAR2DepositorInsertPM-ExRai-AktAR2
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
YFP-Parkin
Plasmid#23955PurposeMammalian expression of human Park2 fused to EYFPDepositorAvailable SinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-63: ACTN2-mEGFP
Plasmid#124607PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassetteDepositorInsertACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only