Addgene

We narrowed to 1,950 results for: CASP

Showing: 501 - 520 of 1950 results

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Nanog-CreER targeting construct

Plasmid

#59720

Purpose

Cre-ER knockin targeting construct for mouse endogenous Nanog locus

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

creERT2

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

mNanog

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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dCas9 fused to scFv (anti-spike)

Plasmid

#186421

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-scFv fusion (anti-SARS-CoV-2 spike)

Use

CRISPR

Tags

6His

Expression

Mutation

Promoter

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_V47N_I48N

Plasmid

#214319

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

V47N_I48N

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_LES33AAA

Plasmid

#214321

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

LES33AAA

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_DDEN29ENGD

Plasmid

#214320

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

DDEN29ENGD

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-human-pro-IL-18_E192K_D193K

Plasmid

#214318

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Retroviral

Tags

Flag and Myc

Expression

Mutation

E192K_D193K

Promoter

MESV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET-20b_HtrA2(delta(133))_S306A_6His

Plasmid

#197975

Purpose

E. coli expression of HtrA2(delta133)-6His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

HtrA2 (HTRA2 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

delta(1-133), S306A

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

Plasmid

#197969

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

L1332V, delta(1616-1666)

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

Plasmid

#197970

Purpose

Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

C328S, C331S, L1332V, delta(1616-1666)

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC-Bio2_MVPI_SMAC(delta(55))_9His

Plasmid

#197978

Purpose

E. coli expression of MVPI-SMAC(delta(55))_9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Use

Tags

9His

Expression

Bacterial

Mutation

delta (1-55), A56M

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC-Bio2_SMAC(delta(55))_Cys_9His

Plasmid

#197977

Purpose

E. coli expression of SMAC(delta(55))_Cys-9His

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

Smac (DIABLO Human)

Use

Tags

9His

Expression

Bacterial

Mutation

delta (1-55), ins240C

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUBI-RZ-Cas12j2

Plasmid

#189781

Purpose

Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Insert

gRNA cloning site

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTU422

Plasmid

#178982

Purpose

13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Inserts

gRAMP
CRISPR array

Use

Tags

Strep-Tag II SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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3389 pcDNA3 Bid p22

Plasmid

#8807

Depositor

Stanley Korsmeyer

Article

Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

Insert

Bid p22 (Bid Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
3392 pcDNA3 Bid p15

Plasmid

#8808

Depositor

Stanley Korsmeyer

Article

Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

Insert

Bid p15 (Bid Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
3' MBD3 TALEN

Plasmid

#52359

Purpose

TALEN vector for targeting human MBD3 locus (3')

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

NN NN HD NN NG NN NI HD HD HD HD NI NN NI HD HD NG

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
5' MBD3 TALEN

Plasmid

#52358

Purpose

TALEN vector for targeting human MBD3 locus (5')

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

NI NN NN NN NN HD HD NI NN NG NG NN NG NN NN NN NN NG NN

Use

TALEN

Tags

Expression

Mutation

Promoter

CAGGS

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
3405 pBM272 Bid p22

Plasmid

#8771

Depositor

Stanley Korsmeyer

Article

Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

Insert

Bid p22 (Bid Mouse)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
Mouse Nanog locus 3' probe

Plasmid

#60037

Purpose

Cloned genomic fragment of murine Nanog locus used as probe for Southern Blot analysis

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

Nanog (Nanog Mouse)

Use

Unspecified; Cloning

Tags

Expression

Mutation

Promoter

Available since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-Myc-PSK1-beta

Plasmid

#197121

Purpose

Expression of MYC-tagged PSK1-beta in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

Insert

TAOK2 variant beta (aa1-1049) (TAOK2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Wild Type variant beta

Promoter

SV40

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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