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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 560 results for: DHFR

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Showing: 501 - 520 of 560 results

  1. SP085

    Plasmid
    #262963
    Purpose
    Barcoded Binding Assay (Barcode 5) with WT Omp25 TMD as the binding sequence
    Depositor
    Matthew Wohlever
    Insert
    3xHA-Sec61β soluble domain-(SH3)5-Omp25TMD-3F4
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    262963-plasmid-map-sequence-id-523597

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  2. SP083

    Plasmid
    #262961
    Purpose
    Barcoded Binding Assay (Barcode 3) with WT Omp25 TMD as the binding sequence
    Depositor
    Matthew Wohlever
    Insert
    3xHA-Sec61β soluble domain-(SH3)3-Omp25TMD-3F4
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    262961-plasmid-map-sequence-id-523595

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  3. SP084

    Plasmid
    #262962
    Purpose
    Barcoded Binding Assay (Barcode 4) with WT Omp25 TMD as the binding sequence
    Depositor
    Matthew Wohlever
    Insert
    3xHA-Sec61β soluble domain-(SH3)4-Omp25TMD-3F4
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    262962-plasmid-map-sequence-id-523596

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  5. cacna2d3 BBS pMT2

    Plasmid
    #206117
    Purpose
    expression of mouse calcium channel alpha2delta-3 with a single Bungarotoxin binding site motif inserted after aa595
    Depositor
    Annette Dolphin
    Insert
    cacna2d3 (Cacna2d3 Mouse)
    Expression
    Mammalian
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206117-plasmid-map-sequence-id-406752

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  6. mEos Cav2.2 pMT2

    Plasmid
    #206092
    Purpose
    expression of rabbit Cav2.2 calcium channel with a N-terminal mEos tag
    Depositor
    Annette Dolphin
    Insert
    cacna1b (CACNA1B Rabbit)
    Tags
    mEos
    Expression
    Mammalian
    Promoter
    Ad MLP/TPL/SV40
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206092-plasmid-map-sequence-id-415462

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  8. pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

    Plasmid
    #117911
    Purpose
    Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM
    Depositor
    Kai Johnsson
    Publication
    Yu et al Science. 2018 Sep 14;361(6407):1122-1126
    Insert
    SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    R44A & H45Q on eDHFR
    Promoter
    T7
    Available since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    117911-plasmid-map-sequence-id-226235

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  17. pMT3-RhoGC D380E

    Plasmid
    #100035
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.
    Depositor
    Daniel Oprian
    Publication
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC D380E
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    D380E
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    100035-plasmid-map-sequence-id-192913

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  18. pMT3-RhoGC D380N

    Plasmid
    #100034
    Purpose
    This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.
    Depositor
    Daniel Oprian
    Publication
    Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1
    Insert
    RhoGC D380N
    Tags
    1D4 (TETSQVAPA)
    Expression
    Mammalian
    Mutation
    D380N
    Promoter
    Adenovirus major late promoter
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    100034-plasmid-map-sequence-id-193102

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  19. pCIS-rPALcc

    Plasmid
    #145804
    Purpose
    expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells
    Depositor
    Betty Eipper, Richard Mains
    Publication
    Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9
    Insert
    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    Expression
    Mammalian
    Mutation
    changed serine 767 to alanine to prevent glycosyl…
    Promoter
    CMV
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    145804-plasmid-map-sequence-id-299336

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Showing: 501 - 520 of 560 results