Addgene

We narrowed to 1,639 results for: Tyr

Showing: 501 - 520 of 1639 results

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pBWB424

Plasmid

#140638

Purpose

RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDB036d

Plasmid

#99216

Purpose

Accessory plasmid for PACE of MjTyrRS variants; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)

Use

Tags

Expression

Bacterial

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp, PproK, and PproA

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB6

Plasmid

#187763

Purpose

MAC-tagged gene expression of human EPHB6

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB6 (EPHB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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HA-RNF34

Plasmid

#119938

Purpose

RING finger protein 34 expression (HA-tag at the N-terminus)

Depositor

Angel de Blas

Article

Jin et al J Biol Chem. 2014 Oct 17;289(42):29420-3

Insert

RNF34 (Rnf34 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only
UAS::PTPRFa.1-DN-GFP

Plasmid

#37110

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRFa.1-DN-GFP (ptprf Zebrafish)

Use

Tol2 gateway expression vector

Tags

EGFP

Expression

Mutation

deleted amino acids 1326-1894

Promoter

UAS-E1b

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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MAC_C_INSR

Plasmid

#187788

Purpose

MAC-tagged gene expression of human INSR

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

INSR (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDB036a

Plasmid

#99215

Purpose

Accessory plasmid for PACE of MjTyrRS variants; negative selection

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)

Use

Tags

Expression

Bacterial

Mutation

amber codons at positions 12 and 203 of T7 RNA po…

Promoter

Ppsp, PproK and PproD

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 CrkII

Plasmid

#50731

Purpose

mammalian expression of CrkII

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII (CRK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pGST-GstZ1

Plasmid

#163090

Purpose

Expressing mouse Gstz1 in bacterial cells

Depositor

Joseph Takahashi

Article

Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

Inserts

GstZ1
GstZ1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I-delta N90

Plasmid

#22165

Depositor

Ananda Roy

Article

Sacristan et al J Biol Chem. 2004 Feb 20. 279(8):7147-58

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

The first 90 aa of TFII-I are deleted

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-2T SHP1 CS

Plasmid

#8595

Depositor

Ben Neel

Article

Timms et al Mol Cell Biol 1998 Jul;18(7):3838-50.

Insert

SHP1 (PTPN6 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C453S (catalytically inactive)

Promoter

Available since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
pLD-hygro-EnVM

Plasmid

#24590

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VM

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
V5-WT-short-UBASH3A

Plasmid

#192088

Purpose

Mammalian expression of human wild-type short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al Diabetes. 2017 Jul;66(7):2033-2043. doi:

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAcBac1-3nitroY-A7-RS

Plasmid

#141173

Purpose

Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

Depositor

Ryan Mehl

Article

Porter et al ACS Chem Biol. 2019 Jun 21;14(6):1328-13

Insert

A7 3nY tRNA synthetase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

P186T L274R N311G C313N

Promoter

CMV

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Use

Tags

Expression

Worm

Mutation

stop at beginning of last exon

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLD-puro-PGKnVA

Plasmid

#24589

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-SLAP-FLAG

Plasmid

#74500

Purpose

Overexpression of SLAP in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al PLoS One. 2012;7(12):e53509. doi: 10.137

Insert

SLAP (SLA Human)

Use

Tags

Expression

Mammalian

Mutation

N18K, G74A, and T149A

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
UAS::PTPRDb-DN-GFP

Plasmid

#37112

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)

Use

Tol2 gateway destination vector

Tags

EGFP

Expression

Mutation

deleted cytodomain, including phosphatase domains…

Promoter

UAS

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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hopscotch

Plasmid

#37284

Depositor

Norbert Perrimon

Article

Binari et al Genes Dev. 1994 Feb 1;8(3):300-12.

Insert

hopscotch (hop Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only