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We narrowed to 2,545 results for: crispr system

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Showing: 501 - 520 of 2545 results

  4. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  9. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54321-plasmid-map-sequence-id-84455

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  11. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Promoter
    xylose
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133316-plasmid-map-sequence-id-266825

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  12. pMG

    Plasmid
    #138264
    Purpose
    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Inserts
    eGFP flanked by iCas9 sites
    mCherry
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tef1
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138264-plasmid-map-sequence-id-273626

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  20. pRhaCAST

    Plasmid
    #211791
    Purpose
    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter
    Depositor
    Silvia Cardona
    Article
    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Rhamnose-inducible
    Available Since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211791-plasmid-map-sequence-id-418380

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Showing: 501 - 520 of 2545 results