We narrowed to 79,939 results for: ELL

1. pHAGE-EF1aL-eGFP-W

   Plasmid

   #126686

   Purpose

   Constitutive, ubiquitous GFP expression.

   Depositor

   Darrell Kotton

   Article

   Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

   Insert

   eGFP
   

   Use

   Lentiviral

   Promoter

   EF1aL

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRESneo-EGFP-alpha Tubulin

   Plasmid

   #12298

   Depositor

   Patricia Wadsworth

   Article

   Rusan et al Mol Biol Cell. 2001 Apr . 12(4):971-80.

   Insert

   EGFP-alpha Tubulin (TUBA1B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSC101_TIMER

   Plasmid

   #103057

   Purpose

   low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. RabV CVS-N2c(deltaG)-APEX2-Venus

   Plasmid

   #73467

   Purpose

   Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   APEX2-Venus
   

   Use

   Neurotropic virus

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pfa6a PhoCl-TsCC(B)::KANMX

   Plasmid

   #185906

   Purpose

   Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   PhoCl-TsCC(B)
   

   Tags

   PhoCl fluorophore with C-terminal TsCC(B)

   Expression

   Yeast

   Promoter

   SP6

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. modRNAc0-Cas9-2A-GFP

   Plasmid

   #170181

   Purpose

   IVT template plasmid for making modified Cas9GFP RNA

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

   Insert

   Cas9-2A-GFP
   

   Use

   Ivt template plasmid

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. RFP-PASS

   Plasmid

   #193971

   Purpose

   Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

   Depositor

   Guangwei Du

   Article

   Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

   Insert

   PA biosenor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pfa6a mScarlet-TsCC(B)::KANMX

   Plasmid

   #176397

   Purpose

   Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

   Depositor

   Matthew Good

   Article

   Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

   Insert

   mScarlet-TsCC(B)
   

   Tags

   mScarlet fused to TsCC(B)

   Expression

   Bacterial and Yeast

   Promoter

   none

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ER-HA-mNG21-10

   Plasmid

   #141165

   Purpose

   Split Fluorescent Reporter for ER Retrograde Trafficking

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   ER-HA-mNG21-10-KDEL
   

   Use

   Lentiviral

   Tags

   Signal Sequence, KDEL and HA

   Expression

   Mammalian

   Mutation

   K128M, S142T, R150M, G172V and K213M

   Promoter

   Ef1a

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PH-PLCD1_mScarlet-H_IRES_SYFP2_PH_N1

    Plasmid

    #85070

    Purpose

    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    PH-PLCD1 (PLCD1 Human)
    

    Tags

    IRES-sYFP2-PH and mScarlet-H

    Expression

    Mammalian

    Mutation

    PLCD aa 1–170

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1-Luciferase

    Plasmid

    #99283

    Purpose

    All-in-one lentivector with tet-inducible luciferase

    Depositor

    Stephen Tapscott

    Article

    Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_GEM4-Halo-FRB

    Plasmid

    #197057

    Purpose

    Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_GEM7-Halo-FRB

    Plasmid

    #197058

    Purpose

    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe-Dvl2-GFP

    Plasmid

    #109148

    Purpose

    Stable Dvl2 expression

    Depositor

    Mariann Bienz

    Article

    Gammons et al J Cell Sci. 2016 Oct 15;129(20):3892-390

    Insert

    Dvl2 (DVL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMP71-tCD19-SIINFEKL

    Plasmid

    #174601

    Purpose

    expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    membrane bound CD19, SIINFEKL (ovalbumin epitope)
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Burden Monitor lambda version

    Plasmid

    #66073

    Purpose

    The burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

    Insert

    GFP
    

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

17. AICSDP-50: NPM1-mEGFP

    Plasmid

    #109122

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human NPM1

    Depositor

    Allen Institute for Cell Science

    Insert

    NPM1 Homology Arms with linker-mEGFP (NPM1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. 408 pSG5L HA E2F1

    Plasmid

    #10736

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    E2F1 (E2F1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. AICSDP-28:TUBA1B-mTagRFP-T

    Plasmid

    #101785

    Purpose

    Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

    Depositor

    Allen Institute for Cell Science

    Insert

    TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFPT-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pEGFP-PCNA-IRES-puro2b

    Plasmid

    #26461

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    PCNA (PCNA Human)
    

    Tags

    pEGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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