We narrowed to 8,583 results for: construct
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Plasmid#65466PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherryDepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialPromoterpl-TetOAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-B
Plasmid#58307PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-C
Plasmid#60979PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic ceruleanDepositorInsertCerulean
UseZebrafishPromoterBactin2Available SinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB-Multibow-O
Plasmid#60982PurposePart of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKODepositorInsertmKO
UseZebrafishPromoterBactin2Available SinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
DH10B E. coli aptI/gidB::Landing Pad
Bacterial Strain#83036PurposeFor site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.DepositorBacterial ResistanceTetracyclineAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHR-FKBP:mCherry-Rab5a
Plasmid#72901PurposeEarly endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab5a (RAB5A Human)
UseLentiviralTagsFKBP(F36V) and mCherryExpressionMammalianMutationfragment a.a. 2-215Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry
Plasmid#182377PurposeDual expression construct encoding 3xFLAG and mCherry from separate promotersDepositorInserts3xFlag
mCherry
ExpressionMammalianPromoterHSV TK promoter and SV40 promoterAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
LI Intron c1
Plasmid#54433DepositorInsertIntron element for construction of silencing vectors (intron1 of AtWRKY33)
UseCloning vectorAvailable SinceJuly 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-BclXLc33
Plasmid#72905PurposeOuter mitochondrial membrane-targeted mCherry-tagged recruiter constructDepositorInsertBl2-like protein 1, isoform Bcl-X(L) (fragment)
UseLentiviralTagsFKBP, mCherryExpressionMammalianMutation33 C-terminal a.a.Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XHA-EGFP-PEX26
Plasmid#139054PurposeHA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XHA-EGFP-PEX26
UseLentiviralTagsHAPromoterUBCAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
12XCSL-d1EGFP
Plasmid#47684PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsd1EGFPMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-G418
Plasmid#160215PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pI4-MTU-DO-HPH
Plasmid#160216PurposeIntegrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMHG-W57* reporter
Plasmid#72850PurposeGFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.DepositorInsertGFP
ExpressionMammalianAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti_dCas9-KRAB-MeCP2_Blast
Plasmid#221501PurposeLentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vectorDepositorInsertdCas9-KRAB-MeCP2-T2A-Blast
UseCRISPR and LentiviralExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-ChrimsonR-mCherry
Plasmid#92207PurposeReporter constructDepositorHas ServiceAAV1InsertChimsonR-mCherry
UseAAVExpressionMammalianPromoterTREAvailable SinceJune 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-mCherry-p2A-eNpHR3.0-TS
Plasmid#158703PurposeReporter constructDepositorInsertmCherry-p2A-eNpHR3.0-TS
UseAAVTagsmCherryExpressionMammalianPromoterTREAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
12XCSL-DsRedExpressDL
Plasmid#47683PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsdsRedExpressDRMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only