We narrowed to 8,179 results for: gnal
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Plasmid#162501PurposeExpresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.DepositorUseTagsGFPExpressionMammalianMutationThe Tac stem region is inserted within ST6Gal1PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only
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GFP-Tac-STC
Plasmid#162490PurposeExpresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
UseTagsGFPExpressionMammalianMutationTac is in partial length with its stem region, TM…PromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
vPyACR_2164382
Plasmid#153029PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_2164382
UseTagseYFPExpressionMammalianMutationvPyACR_2164382 was C-terminally truncated to incl…PromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
vPyACR_21821
Plasmid#153030PurposeAnion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).DepositorInsertvPyACR_21821
UseTagseYFPExpressionMammalianMutationvPyACR_21821 was C-terminally truncated to includ…PromoterCMV (+ enhancer)Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)
Plasmid#123340PurposeNegative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.DepositorInsertVenus-cpVenus-FLARE-EKAR-EV(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)
Plasmid#156412PurposeGateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancerDepositorInsertmurine Dll4 F2- ETS site B/ site #2, 8X
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)
Plasmid#123344PurposeNegative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.DepositorInsertVenus-cpVenus-FLARE-CKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR
Plasmid#123336PurposeCyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.DepositorInsertCerulean3-cpCerulean3-FLARE-AKAR
UseTags6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2(delta)TK-VSV
Plasmid#139353PurposeExpression of TYK2 protein missing the tyrosine kinase (TK) domain (aa 1-895)DepositorInsertTYK2 cDNA expressing aa 1-895 (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-deletion of aa 896-1187PromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBB0405-TEV-His12
Plasmid#137040Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66795.1 (BB_0405 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRevA-TEV-His12
Plasmid#137034Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12DepositorInsertAAF07416.1 (revA Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pP13-TEV-His12
Plasmid#137035Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66426.1 (BB_0034 Borrelia burgdorferi B31)
UseTagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length protein, contains signal p…PromoterT7Available sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
TPC2(E643A)-mCherry
Plasmid#135187PurposeMutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationMutation of glutamate 643 to alaninePromoterAvailable sinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 L211P
Plasmid#124487Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged leucine 211 to prolinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 R312H
Plasmid#124488Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged arginine 312 to histidinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G381R
Plasmid#124490Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged glycine at 381 to argininePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 F416L
Plasmid#124491Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged phenyalanine 416 to leucinePromoterAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-Lyn-AktAR2
Plasmid#125197PurposeSB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2
UseTransposonTagsLyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
hKCNB1 G379R
Plasmid#124489Purposemissense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vectorDepositorInserthuman KCNB1 (KCNB1 Human)
UseTagsHAExpressionMammalianMutationchanged glycine at 379 to argininePromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-PRM-3R
Plasmid#112156PurposeMammalian eGFP expression plasmid for PRM-3R.DepositorInsertPRM-3R (ABL1 Human)
UseTagsExpressionMammalianMutationcontains only the PRM domain of ABl1PromoterCMVAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only