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AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#126217

Purpose

Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-cyan pre-eGRASP(p32)

Plasmid

#111589

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p32) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p32)

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Venus-Flag (865)

Plasmid

#40998

Depositor

Jonathon Pines

Article

Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

Type

Empty backbone

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-NL1B

Plasmid

#15261

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 with insert B (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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integrin beta6 EGFP-N3

Plasmid

#13593

Depositor

Dean Sheppard

Insert

integrin beta 6 (ITGB6 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Paf1

Plasmid

#11059

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Paf1 (PAF1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pC1-HyPerRed-mito

Plasmid

#60247

Purpose

Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HyPerRed-mito

Tags

Mitochondrial targeting signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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sgCtr- LentiCRISPRv2

Plasmid

#107402

Purpose

Lentiviral expression of Cas9 and a control gRNA

Depositor

William Kaelin

Article

Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

Insert

control gRNA

Use

Lentiviral

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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p323-L10A-PATagRFP

Plasmid

#74172

Purpose

Fluorescent reporter for single ribosome tracking

Depositor

Robert Singer

Article

Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

Insert

ribosomal protein L10A (Rpl10a Rat)

Use

Lentiviral

Tags

PATagRFP

Promoter

UbC

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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Cterminal HA WDR59 pRK5

Plasmid

#46328

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR59 (WDR59 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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HA-Mob

Plasmid

#32835

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

Mob (MOB1A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pCFJ68 - Exp5605_Punc-122::GFP::unc-54utr

Plasmid

#19325

Depositor

Erik Jorgensen

Article

Christian et al

Insert

Punc-122::GFP

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG FUCCI

Plasmid

#136934

Purpose

donor plasmid for constitutive FUCCI expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Use

Synthetic Biology

Tags

Clover and mKO2

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-muscle Amphiphysin 2

Plasmid

#22213

Depositor

Pietro De Camilli

Article

Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

Insert

Amphiphysin II (BIN1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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WT lamin A minigene

Plasmid

#20291

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-IGFBP5-V5

Plasmid

#11608

Depositor

Steven Johnson

Insert

Insulin Growth Factor Binding Protein 5 (IGFBP5 Human)

Tags

His and V5

Expression

Mammalian

Mutation

no mutations

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
Rc/CMV cyclin D2 HA

Plasmid

#8950

Depositor

Philip Hinds

Article

Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

Insert

cyclin D2 (CCND2 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pRSFDuet-sumo-NSP12 (SARS-CoV-2)

Plasmid

#159107

Purpose

The SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).

Depositor

Thomas Tuschl

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Insert

NSP12

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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