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pDONR223_PDPK1_WT

Plasmid

#82309

Purpose

Gateway Donor vector containing PDPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PDPK1 (PDPK1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZBTB24_WT

Plasmid

#82887

Purpose

Gateway Donor vector containing ZBTB24, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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G protein alpha-q-CFP

Plasmid

#66081

Purpose

This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

Depositor

Catherine Berlot

Article

Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

Insert

G protein alpha-q-EE-CFP (Gnaq Mouse)

Use

Tags

CFP was inserted internally into the alpha-q sequ…

Expression

Mammalian

Mutation

BamHI and SacI sites in alpha-q were removed with…

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_POU2F2_WT

Plasmid

#81873

Purpose

Gateway Donor vector containing POU2F2 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POU2F2 (POU2F2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-CCNE1

Plasmid

#162553

Purpose

Overexpression of CCNE1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE1 (CCNE1 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_SNAI1_WT

Plasmid

#82164

Purpose

Gateway Donor vector containing SNAI1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SNAI1 (SNAI1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5_C12orf49-miniTurbo

Plasmid

#155112

Purpose

transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

C12orf49 (SPRING1 Human)

Use

Tags

3x Flag and miniTurbo

Expression

Mammalian

Mutation

Promoter

CMV/TO inducible promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_PDCD1LG2_WT

Plasmid

#82287

Purpose

Gateway Donor vector containing PDCD1LG2, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PDCD1LG2 (PDCD1LG2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

F229S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_WT

Plasmid

#82157

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1.shCDK8

Plasmid

#19760

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against CDK8 (CDK8 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pDONR223_PRDM1_WT

Plasmid

#81740

Purpose

Gateway Donor vector containing PRDM1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRDM1 (PRDM1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-BACH1t_RFP

Plasmid

#199214

Purpose

BACH1 C-terminus truncated isoform - BACH1t expression plasmid with a RFP reporter on the backbone.

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Inserts

BACH1t (BACH1 Human)
RFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and PGK

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAC1_p.Q61L

Plasmid

#82178

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Q61L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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retro-gfp-puro vector

Plasmid

#58249

Purpose

Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKACB_WT

Plasmid

#82224

Purpose

Gateway Donor vector containing PRKACB , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKACB (PRKACB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TRCN0000338399

Plasmid

#78156

Purpose

shXPO1 targeting CDS

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

XPO1 (XPO1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

hU6 and hU6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_SLC2A3_WT

Plasmid

#81787

Purpose

Gateway Donor vector containing SLC2A3 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RIT1_WT

Plasmid

#82882

Purpose

Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

Depositor

David Root, Matthew Meyerson, Jesse Boehm

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RIT1 (RIT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF804B

Plasmid

#88756

Purpose

Donor Vector containing ZNF804B transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF804B (ZNF804B Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Last nucleotide of stop codon removed to allow fo…

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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YAP1-Y357F-V5 in pLX304

Plasmid

#42556

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Y357F

Promoter

CMV

Available since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T2-Ste7-EE

Plasmid

#52681

Purpose

expression of constitutively active Ste7 kinase in bacteria

Depositor

Benjamin Turk

Article

Mok et al Sci Signal. 2010 Feb 16;3(109):ra12. doi

Insert

Ste7 (STE7 Budding Yeast)

Use

Tags

GST and Myc

Expression

Bacterial

Mutation

S359E/T363E constitutively active

Promoter

tac

Available since

April 30, 2014

Availability

Academic Institutions and Nonprofits only
pXPR_023d-sgCiSF3B1

Plasmid

#202446

Purpose

Expression of a CRISPRi guide targeting SF3B1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

SF3B1 gRNA (SF3B1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP6C_p.R264C

Plasmid

#81341

Purpose

Gateway Donor vector containing PPP6C , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP6C (PPP6C Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R264C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNFAIP3_WT

Plasmid

#82238

Purpose

Gateway Donor vector containing TNFAIP3 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFAIP3 (TNFAIP3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PRKCZ_WT

Plasmid

#82228

Purpose

Gateway Donor vector containing PRKCZ , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCZ (PRKCZ Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SMAD4_p.R361H

Plasmid

#81510

Purpose

Gateway Donor vector containing SMAD4 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R361H

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_IRGM_WT

Plasmid

#82134

Purpose

Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRGM (IRGM Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_SMURF2_WT

Plasmid

#82259

Purpose

Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMURF2 (SMURF2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_sgFLNB-SK2

Plasmid

#119873

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK2 (FLNB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_sgFLNB-SK4

Plasmid

#119874

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK4 (FLNB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRosa26-EF1a-hCas9IRESneo

Plasmid

#67987

Purpose

Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-hCas9IRESneopA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RBM10_WT

Plasmid

#81771

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_PNKP_WT

Plasmid

#81801

Purpose

Gateway Donor vector containing PNKP , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PNKP (PNKP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX304-MARK3

Plasmid

#107235

Purpose

Lentiviral gene expression vector for phospho-MARK3 expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_POT1_WT

Plasmid

#81820

Purpose

Gateway Donor vector containing POT1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

POT1 (POT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223_RAC1_p.T17N

Plasmid

#82179

Purpose

Gateway Donor vector containing RAC1 , part of the Target Accelerator Plasmid Collection.

Depositor

David Root, William Hahn, Jesse Boehm

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAC1 (RAC1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

T17N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
peGFP-N1-Cofilin

Plasmid

#186747

Purpose

Expresses wildtype GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-RAP1A-E63-puro

Plasmid

#156167

Purpose

Lentiviral vector for expression of RAP1A-E63, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK_Hyg_mTurquoise2_Atg4BC74A

Plasmid

#161733

Purpose

Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

mTurquoise2

Expression

Mammalian

Mutation

220-222 TGC (Cys) is changed to GCA (Ala)

Promoter

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

Plasmid

#161923

Purpose

To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting HSD17B11 exon 1

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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