We narrowed to 7,266 results for: 11

1. pAAV-EF1α1.1-Chronos-tdTomato

   Plasmid

   #122099

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α (1.1 kb short version)

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

   Plasmid

   #108273

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α promoter (1.1kb short version)

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1α1.1-FLPX-rc [Chronos-tdTomato]

   Plasmid

   #122103

   Purpose

   AAV-mediated expression of Chronos-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1α1.1-FLEX-rc [Chronos-GFP]

   Plasmid

   #84485

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α, 1.1 kb long (short version)

   Available since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

   Plasmid

   #114080

   Purpose

   CAG promoter expression plasmid for human codon optimized AsBE1.4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
   

   Use

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

   Plasmid

   #114077

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   E174R/S542R/K607R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CAG-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #118295

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the CAG promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CAG

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #128588

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)

   Plasmid

   #114083

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.3

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid

    #114071

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    

    Use

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R and S542R

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid

    #114079

    Purpose

    CAG promoter expression plasmid for human codon optimized AsBE1.1

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AURKA-Aquamarine

    Plasmid

    #228564

    Purpose

    Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

    Depositor

    Giulia Bertolin

    Article

    Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

    Insert

    AURKA (AURKA, AURKA Human)
    

    Use

    Tags

    Aquamarine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. VEGF120TAG

    Plasmid

    #200946

    Purpose

    Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

    Depositor

    Elizabeth Kirby

    Article

    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

    Inserts

    VEGF120TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    

    Use

    Lentiviral

    Tags

    6x HIS

    Expression

    Mutation

    amber stop codon 101 bp from ATG

    Promoter

    Ef1a and hPGK

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. VEGF164TAG

    Plasmid

    #200947

    Purpose

    Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

    Depositor

    Elizabeth Kirby

    Article

    Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

    Inserts

    VEGF164TAG (Vegfa Mouse)
    hPGK-driven Puromycin resistance
    

    Use

    Lentiviral

    Tags

    6x HIS

    Expression

    Mutation

    amber stop codon 101 bp from ATG

    Promoter

    Ef1a and hPGK

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-GFP-LA

    Plasmid

    #206197

    Purpose

    Expresses GFP-tagged human Lamin-A protein by the constitutive CMV promoter in mice

    Depositor

    Yuxuan Guo

    Article

    Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

    Insert

    Lmna (LMNA )
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSPL3-hRPH3A_c.1853Awt

    Plasmid

    #190478

    Purpose

    minigene assay

    Depositor

    Alfredo Brusco

    Article

    Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

    Insert

    RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HisGB1-Fusion + CBP TAZ1(340-439)

    Plasmid

    #173762

    Purpose

    Bacterial coexpression of CBP TAZ1 and CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HisGB1-FusionL21A + CBP TAZ1(340-439)

    Plasmid

    #173763

    Purpose

    Bacterial Coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246L231A):Hif1a(796-826) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HIsGB1-FusionL63A + CBP TAZ1(340-439)

    Plasmid

    #173764

    Purpose

    Bacterial coexpression of CBP TAZ1 with CITED2/Hif1a activation domain constructs

    Depositor

    Peter Wright

    Article

    Appling et al Structure. 2021 Sep 11. pii: S0969-2126(

    Inserts

    CITED2(216-246):Hif1a(796-826L822A) (HIF1A, CITED2 Human)
    CBP (340-439) (Crebbp Mouse)
    

    Use

    Tags

    His6GB1 and thrombin

    Expression

    Bacterial

    Mutation

    fusion of CITED2 and Hif1a

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGMC00001

    Plasmid

    #166718

    Purpose

    sgRNA against human BAP1

    Depositor

    Raj Chari

    Article

    Rathkey et al J Thorac Oncol. 2020 May;15(5):843-859.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge