We narrowed to 11,726 results for: EMB

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EGFP G protein Gamma 3(K68A,K69A,F70A,F71A) in pcDNA3.1

Plasmid

#42189

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Gamma 3(K68A,K69A,F70A,F71A) (Gng3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

K68A,K69A,F70A,F71A

Promoter

CMV

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-MBP-ULK1-1-835-mcherry

Plasmid

#249729

Purpose

Expressing human ULK1-1-835 with a MBP tag in N-terminal and a mcherry tag in C-terminal

Depositor

James Hurley

Article

Duan et al bioRxiv [Preprint]. 2025 Nov 9:2025.11.0

Insert

ULK1 (ULK1 Human)

Tags

MBP and Mcherry

Expression

Mammalian

Mutation

1-835

Promoter

CAG

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Jarid2-polyA

Plasmid

#223811

Purpose

IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

Depositor

Azusa Inoue

Article

Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

Insert

Jarid2 (Jarid2 Mouse)

Use

In vitro transcription

Tags

Flag

Expression

Mammalian

Promoter

CMV, T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-Halo-Tag

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SmBiT

Plasmid

#223618

Purpose

Lentiviral expression of human CD86 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD86

Plasmid

#223598

Purpose

Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(FL)

Plasmid

#221243

Purpose

Expression of STING without transmembrane domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING without transmembrane domain (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGM8

Plasmid

#220166

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TSPO (TSPO Human)

Use

Lentiviral

Tags

GFP11

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA5-PSMA6-PSMA7

Plasmid

#217894

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA5 (PSMA5 Human)
PSMA6 (PSMA6 Human)
PSMA7 (PSMA7 Human)

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDM1383

Plasmid

#207423

Purpose

Extrachromosomal expression HSPC300-GFP and mCherry-Hs Raf1 (1–134 RBD)

Depositor

Rob Kay

Article

Veltman et al Elife. 2016 Dec 13;5:e20085. doi: 10.755

Inserts

HSPC300
Raf1 (RAF1 Human)

Use

Unspecified

Tags

GFP and mCherry

Mutation

aa 1–134 (RBD) only

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-GBF1(E794K)

Plasmid

#206416

Purpose

Mammalian expression of hGBF1(E794K)

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Tags

HA

Expression

Mammalian

Mutation

E794K

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-CRY2-mCh-superPLDx30

Plasmid

#188988

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgMUC4

Plasmid

#198399

Purpose

Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

MUC4 sgRNA (MUC4 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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SNAP-CLC-EGFP

Plasmid

#193579

Purpose

Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

SNAP-tag and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_mApple-SEPT7 Gmut2

Plasmid

#180317

Purpose

mammalian expression of human SEPT7 Gmut2 fused to mApple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

mApple

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i3-mApple

Plasmid

#180323

Purpose

mammalian expression of human SEPT9_i3 fused to monomeric Apple

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v3 (SEPTIN9 Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG1_zeo

Plasmid

#184910

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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C413-E45: attB4-hr5-ie1p>-attB1r

Plasmid

#162930

Purpose

Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV ie1 promoter with hr5 enhancer

Use

Synthetic Biology

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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