We narrowed to 22,823 results for: Sis

1. pET30(a)-AtNUDX23

   Plasmid

   #175021

   Purpose

   Purification of AtNUDX23 enzyme

   Depositor

   Jeppe Vinther

   Article

   Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

   Insert

   AtNUDX23 (NUDX23 Mustard Weed)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2-sgPnpt1-1#

   Plasmid

   #234769

   Purpose

   knockout Pnpt1

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Pnpt1 (Pnpt1 )
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. PMXS-GOT1

   Plasmid

   #72872

   Purpose

   The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   GOT1 (GOT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   sgRNA resistant human GOT1, 7 silent mutations c1…

   Promoter

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pCJ208

   Plasmid

   #162681

   Purpose

   Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of W185-F191 with G251-T472 from MHET…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCJ191

   Plasmid

   #162668

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCJ217

   Plasmid

   #162685

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   G489C, S530C; codon optimized for E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCJ209

   Plasmid

   #162682

   Purpose

   Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of G251-T472 with W185-F191 from PETa…

   Promoter

   T7/lac

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCJ206

   Plasmid

   #162679

   Purpose

   pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   E226T; codon optimized for expression in E. coli …

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pABD1275

   Plasmid

   #68017

   Purpose

   PIF1 protein overproduction for purification from bacterial cells

   Depositor

   Bruce Downie

   Article

   Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

   Insert

   PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Codon Optimized for bacterial expression (GenScri…

   Promoter

   T7

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

10. CLV1 L2_pECIA2

    Plasmid

    #115100

    Purpose

    Bait vector CLV1 L2_pECIA2 should be used with prey vector CLV1 L2_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G75820 (CLV1 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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