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pFS_0370_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1_RC

Plasmid

#116982

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0371_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_2

Plasmid

#116983

Purpose

Expresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Azospirillum lipoferum 4B RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pXT

Plasmid

#107574

Purpose

Contains the VGF promoter (about 700 bp) plus 800 bp of 5' untranslated sequence. The HindIII (5')-HincII(3') fragment acts as a NGF-inducible promoter

Depositor

Andrea Levi

Article

Possenti et al Proc Natl Acad Sci U S A. 1992 May 1;89(

Insert

VGF promoter (Vgf Rat)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PP258

Plasmid

#78962

Purpose

Promoter: GAP6, minimal Promoter: mCYC. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP310

Plasmid

#78979

Purpose

245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP152

Plasmid

#78986

Purpose

Supplemental Figure 4. Promoter long ppTEF1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP153

Plasmid

#78997

Purpose

Supplemental Figure 4. Promoter GAP7. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP362

Plasmid

#78981

Purpose

255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
36INS-pTet_GB

Plasmid

#78681

Purpose

Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_R0040. [G:36INS-pTet:B]

Depositor

Douglas Densmore

Article

Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do

Insert

Insulated pTet Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP87

Plasmid

#78987

Purpose

Supplemental Figure 4. Promoter scTEF1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP263

Plasmid

#78972

Purpose

Promoter: scTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP261

Plasmid

#78973

Purpose

Promoter: GAP, minimal Promoter: GAP6. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP262

Plasmid

#78974

Purpose

Promoter: GAP6, minimal Promoter: GAP6. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP254

Plasmid

#78975

Purpose

Promoter: GAP6, minimal Promoter: GAP. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP253

Plasmid

#78976

Purpose

Promoter: GAP, minimal Promoter: GAP. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP229

Plasmid

#78977

Purpose

Promoter: ppTEF1, minimal Promoter: GAP6. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP311

Plasmid

#78980

Purpose

246R. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP326

Plasmid

#78983

Purpose

245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

rHGH
IFNa2b

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP247

Plasmid

#78964

Purpose

Promoter: scTEF1, minimal Promoter: AOX1. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PP276

Plasmid

#78970

Purpose

Promoter: GAP6, minimal Promoter: GCW14. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge