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pAAV-hGRM1-RFP

Plasmid

#22920

Depositor

Edward Callaway

Publication

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)

Use

AAV

Expression

Mammalian

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pAS167

Plasmid

#194490

Purpose

37-12 Op (Part 2)

Depositor

Ahmad Khalil

Publication

Sanford et al ACS Synth Biol. 2022 Nov 11. doi: 10.102

Insert

37-12 Op (Part 2)

Use

Synthetic Biology

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Gal ts-lux

Plasmid

#1329

Depositor

Susan Lindquist

Publication

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

bacterial luciferase-V.h.MAV

Expression

Yeast

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
mCherry-NLS-mCherry

Plasmid

#231890

Purpose

mCherry-tagged nuclear rupture marker

Depositor

Lukas Kapitein

Publication

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

mCherry-SV40 NLS-mCherry

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pKH115_GALNT2-keima

Plasmid

#214969

Purpose

Golgiphagy flux reporter GALNT2-keima

Depositor

Wade Harper

Publication

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GALNT2-keima (GALNT2 Human)

Use

Lentiviral

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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mito-EcSTH-LbNOX

Plasmid

#211922

Purpose

Mitochondrial localization of EcSTH-LbNOX

Depositor

Binghui Li

Publication

Yang et al Nat Commun. 2022 Nov 17;13(1):7031. doi:

Insert

mito-EcSTH-LbNOX

Use

Lentiviral

Tags

3xFLAG

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pPIPL12-FnCas12a

Plasmid

#199563

Purpose

Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

Depositor

Philippe Lambin

Publication

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

Fncas12a

Use

CRISPR and Synthetic Biology; Tetracycline induci…

Expression

Bacterial

Promoter

COtetR-PIPL12

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon

Plasmid

#206218

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Publication

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX303 GFP-PIK3C2A

Plasmid

#162000

Purpose

Lentiviral expression vector containing PIK3C2A tagged with GFP

Depositor

David Bryant

Publication

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Human)

Use

Lentiviral

Tags

eGFP

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21ΔRING-Box

Plasmid

#105520

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

Depositor

Melina Schuh

Publication

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Mutation

deletion of RING domain (aa124-462)

Promoter

T7

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Publication

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Expression

Mammalian

Promoter

CBA

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pICH47742:FCP:Cas9YFP

Plasmid

#85986

Purpose

Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

Depositor

Thomas Mock

Publication

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Insert

Cas9

Use

CRISPR; Golden gate assembly

Tags

SV40 NLS, YFP

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pExp_CAG:EYFP

Plasmid

#51791

Purpose

Constitutively expresses yellow fluorescent protein , EYFP

Depositor

Ron Weiss

Publication

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

EYFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 S204A

Plasmid

#27114

Depositor

Joan Massague

Publication

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

S204A

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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vAS1

Plasmid

#198745

Purpose

3xHA-3'UTRmg105 -pmg153-NAT

Depositor

Chantal Abergel

Publication

Philippe et al Nat Protoc. 2023 Nov 14. doi: 10.1038/s4

Type

Empty backbone

Use

Endogenous tagging of mimivirus/megavirus genes

Tags

HA tag

Promoter

pmg741

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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ABHD16A-mNG

Plasmid

#195157

Purpose

mammalian expression of ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Publication

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.VAMP2-GFP-SBP

Plasmid

#180249

Purpose

Mammalian expression of VAMP2

Depositor

Marvin Bentley

Publication

Nabb et al Mol Biol Cell. 2021 Nov 3:mbcE21080425.

Insert

VAMP2 (Vamp2 Rat)

Expression

Mammalian

Mutation

WT

Promoter

Chicken Beta actin

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T1 R1276P (+minintr, KDR)

Plasmid

#119202

Purpose

transfer vector

Depositor

Helen Morrison

Publication

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 1 mini-gene (NF1 Human)

Use

Transfer vector

Mutation

R1276P

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T2 R1276P (+minintr, KDR)

Plasmid

#119203

Purpose

transfer vector

Depositor

Helen Morrison

Publication

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Mutation

R1276P

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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