Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 22,619 results for: his

Showing: 5221 - 5240 of 22619 results

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pETDuet-His-SUMO-TEV-NpuAK1AK3A

Plasmid

#257738

Purpose

For expression and co-expression with the modifying enzyme in the pathway, NpuN (acetyltransferase)

Depositor

Wilfred van der Donk

Article

Weir et al Biochemistry. 2026 May 5;65(9):1578-1591

Insert

NpuA

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

K1A, K3A

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-His-SUMO-NpuAS4A-NpuM

Plasmid

#257739

Purpose

For expression to understand where dehydrations occurred

Depositor

Wilfred van der Donk

Article

Weir et al Biochemistry. 2026 May 5;65(9):1578-1591

Insert

NpuA, NpuM

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

NpuA S4A

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-His-SUMO-NpuA-NpuM

Plasmid

#257735

Purpose

For expression to understand PTM and characterized

Depositor

Wilfred van der Donk

Article

Weir et al Biochemistry. 2026 May 5;65(9):1578-1591

Insert

NpuA, NpuM

Tags

6xHis-SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-His-SUMO-TEV-NpuAK1A

Plasmid

#257736

Purpose

For expression and co-expression with the modifying enzyme in the pathway, NpuN (acetyltransferase)

Depositor

Wilfred van der Donk

Article

Weir et al Biochemistry. 2026 May 5;65(9):1578-1591

Insert

NpuA

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

K1A

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pETDuet-His-SUMO-TEV-NpuAK3A

Plasmid

#257737

Purpose

For expression and co-expression with the modifying enzyme in the pathway, NpuN (acetyltransferase)

Depositor

Wilfred van der Donk

Article

Weir et al Biochemistry. 2026 May 5;65(9):1578-1591

Insert

NpuA

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

K3A

Promoter

T7

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA6-myc-BKαΔM-GFP-V5-His

Plasmid

#255910

Purpose

Complementary plasmid for co-expression with BKαΔM module to form functional BK channels. BKα residues 94–651 are replaced by a flexible peptide linker and GFP is tagged at C-terminus.

Depositor

Jiusheng Yan

Article

Chen et al Elife. 2026 Mar 5;14:RP107681. doi: 10.7

Insert

KCNMA1 (partial) (KCNMA1 Human)

Tags

EGFP-V5-6xHis and Myc

Expression

Mammalian

Mutation

residues 94–651 are deleted and replaced by a fle…

Available Since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I44A

Plasmid

#253393

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I44A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44A

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I120A

Plasmid

#253394

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I120A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I120A

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I44AI120A

Plasmid

#253395

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I44AI120A double mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44AI120A

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAJS1228 His-Sumo-Tau-1-172

Plasmid

#250751

Purpose

Vector for bacterial expression of tau 1-172 with scarless SMNT3/Ulp1 cleavage

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Insert

Tau (MAPT Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

aa 1-172 only

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAJS1229 His-Sumo-Tau-1-176

Plasmid

#250752

Purpose

Vector for bacterial expression of tau 1-176 with scarless SMNT3/Ulp1 cleavage

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Insert

Tau (MAPT Human)

Tags

6xHis-Sumo

Expression

Bacterial

Mutation

aa 1-176 only

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAJS1085 pET His-Sumo-ORF

Plasmid

#250758

Purpose

Vector for bacterial expression of proteins with scarless SMNT3/Ulp1 cleavage

Depositor

Martin Kampmann

Article

Samelson et al Cell. 2026 Jan 28:S0092-8674(25)01487-4.

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mDE-Myc-His

Plasmid

#249180

Purpose

AAV mediated overexpression of mouse Sparcl1 a.a. 330-550 fragment

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mSLF-Myc-His

Plasmid

#249179

Purpose

AAV mediated overexpression of C-terminal fragment of mouse Sparcl1

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Mutation

C-terminal fragment

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-mHevin-Myc-His

Plasmid

#249178

Purpose

AAV mediated overexpression of full length mouse Sparcl1

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Use

AAV

Tags

6xHis and Myc

Expression

Mammalian

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP-Tag5-IgKsp-DE-Myc-His

Plasmid

#249176

Purpose

Overexpression of mouse Sparcl1 fragment a.a. 330-550 with Myc and 6xHis tag

Depositor

Cagla Eroglu

Article

Ramirez et al Neuron. 2026 Feb 19:S0896-6273(25)00984-

Insert

Sparcl1 (Sparcl1 Mouse)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

amino acids 330–550

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-sGPR124 D364E-His

Plasmid

#246711

Purpose

Expression vector for secreted human GPR124 ECD D364E (RGD -> RGE), N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD D364E

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-GPR124 LRR-His

Plasmid

#246695

Purpose

Expression vector for secreted human GPR124 LRR domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

LRR domain

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-sGPR124 ΔGAIN-His

Plasmid

#246693

Purpose

Expression vector for secreted human GPR124 ECD lacking the GAIN domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 427-755

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xFLAG-CMV-9-sGPR124 ΔHRM-His

Plasmid

#246692

Purpose

Expression vector for secreted human GPR124 ECD lacking the HRM domain, N-terminal 3xFLAG tag, C-terminal 6xHis tag

Depositor

Calvin Kuo, Mario Vallon

Article

Vallon et al Cell Rep. 2018 Oct 9;25(2):339-349.e9. d

Insert

GPR124 (ADGRA2 Human)

Tags

3xFLAG and 6xHis

Expression

Mammalian

Mutation

ECD Δaa 349-426

Promoter

CMV

Available Since

Dec. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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