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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Expression

Yeast

Promoter

FBA1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCR-BluntII-TOPO-RhoC

Plasmid

#14099

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

Human RhoC (RHOC Human)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
CYPHR

Plasmid

#14864

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

cyan/yellow PH domain reporter (Plcd1 )

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
KW63_HART_RAC1_PuroR

Plasmid

#207400

Purpose

Lentiviral plasmid containing DDX3X reporter HART for RAC1 5UTR - PuroR

Depositor

Stephen Floor

Article

Wilkins et al RNA. 2024 Jul 16;30(8):1041-1057. doi: 1

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-Fn-crRNA

Plasmid

#78958

Purpose

Cloning vector for expression of FnCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPS2351

Plasmid

#1352

Depositor

Pamela Silver

Article

Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

Insert

MGA2 (MGA2 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pJW1234

Plasmid

#62367

Purpose

pRS305 pPGK1-BirA::LEU2

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA (birA )

Expression

Yeast

Promoter

PGK

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-TLG1

Plasmid

#105261

Purpose

Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg1 (TLG1 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pPS1739

Plasmid

#8922

Depositor

Pamela Silver

Article

Ferrigno et al EMBO J. 1998 Oct 1. 17(19):5606-14.

Insert

HOG1 (HOG1 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pESC-URA-Rnq1-GFP

Plasmid

#21051

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
6974 pPROEX-HTb-SSB1

Plasmid

#1232

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ssb1 (SSB1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pPS2348

Plasmid

#1351

Depositor

Pamela Silver

Article

Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

Insert

SPT23 (SPT23 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pWD509

Plasmid

#73739

Purpose

Gateway compatible [1-2] Entry Vector encoding 2xNLS-FLP-D5

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJJH1260

Plasmid

#36913

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Inserts

ScURA3
ScURA3

Expression

Bacterial, Mammalian, and Y…

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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N113 nLV EF-1a-Gal4-Stop-PGK-Puro

Plasmid

#44176

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Gal4/DBD (1-147)

Use

Lentiviral

Promoter

EF-1a

Available Since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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CUP Cyc8 YFP p316

Plasmid

#15594

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

CYC8 (CYC8 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
PE-maturase-RT

Plasmid

#181807

Purpose

PE-E.r. maturase RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

PE-maturase-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSB305-K3L-vac

Plasmid

#20053

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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YIplac204-TC-OM45-FKBPx4

Plasmid

#104472

Purpose

Integrating plasmid for overexpression of OM45-FKBPx4.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

OM45p (OM45 Budding Yeast)

Tags

FKBPx4- HA

Expression

Yeast

Promoter

TPI1

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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