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Showing: 5261 - 5280 of 18384 results

  1. pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53206
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    John Pringle
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and TRP1
    Promoter
    GAL1 promoter
    Available Since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pTXB1-CRP/CAP

    Plasmid
    #51563
    Purpose
    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    CRP/CAP
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51563-plasmid-map-sequence-id-78803

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  3. pDON-AI-baIaa

    Plasmid
    #51474
    Purpose
    Express mouse TH2B and TH2A in mammalian cells
    Depositor
    Shunsuke Ishii
    Inserts
    histone cluster 1, H2aa (H2ac1 Mouse)
    histone cluster 1, H2ba (H2bc1 Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Available Since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51474-plasmid-map-sequence-id-78546

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  4. EIF4A2

    Plasmid
    #25259
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    eIF4A2:G22-E240 (EIF4A2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids G22-E240
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. 3xFLAG-dCas9/pMSCVhyg

    Plasmid
    #134323
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134323-plasmid-map-sequence-id-430622

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  8. p663-UBC-miniTurbo-V5-NEK6_IDG-K

    Plasmid
    #189872
    Purpose
    Gateway destination clone of NEK6 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK6 (NEK6 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189872-plasmid-map-sequence-id-374315

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  11. p667-UBC-CDK12-V5-miniTurbo_IDG-K

    Plasmid
    #135243
    Purpose
    Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    CDK12-V5-miniTurbo (CDK12 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135243-plasmid-map-sequence-id-269114

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  12. Lamin A (R527P)-mRFP

    Plasmid
    #124272
    Purpose
    Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R527P) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R527P)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124272-plasmid-map-sequence-id-241977

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  16. pcDNA_DiCre2_60.F2

    Plasmid
    #60210
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(60-343)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 60-343 …
    Promoter
    CMV
    Available Since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60210-plasmid-map-sequence-id-96296

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  18. pLenti4/V5-DEST-P85(G376R)

    Plasmid
    #40220
    Depositor
    Lynda Chin
    Insert
    P85(G376R) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    changed Glycine 376 to Arginine
    Promoter
    CMV
    Available Since
    Oct. 22, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 5261 - 5280 of 18384 results