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pGEX Slp76-SH2

Plasmid

#46503

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slp76 (LCP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 417-533

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pD2sE_SC10

Plasmid

#219716

Purpose

DV2 stable E dimer with fusion loop mutation

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC10

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

G106D, A259W, T262R, F279W, T280P

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_FNDC3B

Plasmid

#45269

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

FNDC3B (FNDC3B Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Lyn-SH2

Plasmid

#46452

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lyn (LYN Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 106-213

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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KAT6B-luciferase

Plasmid

#113353

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

KAT6B-enhancer (KAT6B Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Stat1-SH2

Plasmid

#46511

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat1 (STAT1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 488-683

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Hck-SH2

Plasmid

#46445

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Hck (HCK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 134-254

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Fer-SH2

Plasmid

#46425

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fer (FER Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 450-558

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS8/myc-His

Plasmid

#127489

Purpose

Expresses myc-tagged NDUFS8 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS8 (NDUFS8 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F1 3'UTR

Plasmid

#29468

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F1 3'UTR (E2F1 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCEP4-myc-GRM3-VC

Plasmid

#98968

Purpose

myc-tagged human mGluR3 fused to C-terminus of split Venus

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

GRM3 (GRM3 Human)

Tags

Signal/leader sequence from HLA class I A-2 alpha…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA3 CTF-FLAG

Plasmid

#217680

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRA3 (ADGRA3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-737

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRA2 CTF-FLAG

Plasmid

#217679

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRA2 (ADGRA2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-746

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCK668

Plasmid

#192637

Purpose

Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dCas9-NG ha…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-p27Kip1 3'UTR

Plasmid

#29476

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

p27 Kip1 3'UTR (CDKN1B Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-Blast SHMT2 CD

Plasmid

#106302

Purpose

Expresses SHMT2 K280A catalytic site mutant

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K280A catalytic site mutation, Silent mutations d…

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-iGluSnFR.S72A

Plasmid

#106194

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

GFAP

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV NucleoBAS N354K-I372V

Plasmid

#63574

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS N354K-I372V

Tags

SV40 NLS

Expression

Mammalian

Mutation

N345K and I372V

Promoter

CMV

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

Plasmid

#138525

Purpose

Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

Depositor

Richard Jenner

Article

Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

Insert

Control non-PRC2 binding A-tract repeat RNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCIBN-tagBFP-TetR

Plasmid

#103797

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-tagBFP-TetR (CIB1, tetR Mustard Weed, E. coli)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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