We narrowed to 12,110 results for: NSI
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Plasmid#204470PurposeThis is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.DepositorInsertsUseSynthetic BiologyMutationLasR L128F-S129W mutationPromoterIn an operon and PLasAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only
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pCS2+ HA-BEX1
Plasmid#231002PurposeTransient overexpression of human BEX1 in mammalian cellsDepositorAvailable SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-AP4-cyto
Plasmid#229694PurposeTransient expression of EGFP AP4-cyto in mammalian cellsDepositorInsertEGFP; Control construct with tandem AP4 motifs (cytoplasm)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-FP4-cyto
Plasmid#229695PurposeTransient expression of FP4-cyto in mammalian cellsDepositorInsertEGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4668
Plasmid#215272PurposeModule for the expression of the N. nambi HISPS gene under the ABA- responsive MAPKKK18 promoter and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertpMAPKKK18:HISPS.TNOS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNACs):GFP
Plasmid#218570PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationMutation genomic sequence 85nt, 86nt from TA to C…Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
Plasmid#211847PurposePlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degronDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoter35SAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48)-VSV
Plasmid#212742PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-VSV
Plasmid#212741PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-CUbo-VSV
Plasmid#212733PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-VSV
Plasmid#212732PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-VSV
Plasmid#212729PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-VSV
Plasmid#212728PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-PTS1
Plasmid#209398PurposeTransiently expressing TurboID fused with a peroxisomal targeting signal type I (PTS1) in plantaDepositorInsert3XHA-TurboID-PTS1
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GTS-TurboID
Plasmid#209397PurposeTransiently expressing TurboID fused with a Golgi targeting sequence (GTS) in plantaDepositorInsertGTS-3XHA-TurboID
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LLFLS2-2
Plasmid#202188PurposeExpress 'Frost Lisbon' Lemon FLS2-2 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
TagsHAExpressionPlantPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LLFLS2-1
Plasmid#202187PurposeExpress 'Frost Lisbon' Lemon FLS2-1 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
TagsHAExpressionPlantPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-RCaMP1h
Plasmid#191467PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent proteinDepositorInsertRCaMP1h
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VC155
Plasmid#194046PurposeTransient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
TagsAtOEP7 1–50aaExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VN154
Plasmid#194045PurposeTransient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
TagsAtOEP7 1–50aaExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB2890
Plasmid#193133PurposeA2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.DepositorInsertGB_SynP (A2) G1dG2b.1
UseSynthetic BiologyAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y
Plasmid#191459PurposeCompare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent proteinDepositorInsertmTurquoise2_T203Y
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-GCaMP3
Plasmid#191460PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) GCaMP3 to mScarlet-I fluorescent proteinDepositorInsertGCaMP3
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-K-GECO1
Plasmid#191469PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) K-GECO1 to mTurquoise2 fluorescent proteinDepositorInsertK-GECO1
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-R-GECO1
Plasmid#191471PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) R-GECO1 to mTurquoise2 fluorescent proteinDepositorInsertR-GECO1
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOP004
Plasmid#185985PurposeEncodes a 0.8 kb bacterial transcription unit with Broccoli-tRNA fluorogenic aptamer shortly upstream of the intrinsic terminatorDepositorInsertBacterial transcription unit encoding Broccoli-tRNA fluorogenic aptamer
ExpressionBacterialAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-CH-GECO2.1
Plasmid#191470PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) CH-GECO2.1 to mTurquoise2 fluorescent proteinDepositorInsertCH-GECO2.1
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-jGCaMP7c
Plasmid#191461PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jGCaMP7c to mScarlet-I fluorescent proteinDepositorInsertjGCaMP7c
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut
Plasmid#178910Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseMutationCTG>AGT and TGC>CAT mir16+153 mutatedPromoterSV40 promoterAvailable SinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY251
Plasmid#184153PurposeReprogrammed short tracrRNA for hijacking mRNA of arsenic responsive gene cluster (site 2)DepositorInserts-tracrRNA-Ar2
UseSynthetic BiologyExpressionBacterialPromoteraraBAD promoterAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-sfGFP-SKL-Target2 (Mpphot)
Plasmid#186722PurposeTransient expression vector for SKLPT imaging for target 2 (Mpphot) in plant cells (Negative control vector).DepositorInsertsfGFP-SKL-Mphot
ExpressionBacterialAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-sfGFP-SKL-Target1 (Mpphot)
Plasmid#186721PurposeTransient expression vector for SKLPT imaging for target 1 (Mpphot) in plant cells (Positive control vector).DepositorInsertsfGFP-SKL-Mphot
ExpressionBacterialAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-sgRNA_Target2 (Mpphot)
Plasmid#186727PurposeGateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.DepositorInsertsgRNA_Mphot
ExpressionBacterialAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-sgRNA_Target1 (Mpphot)
Plasmid#186726PurposeGateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.DepositorInsertsgRNA_Mphot
ExpressionBacterialAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16-mut1
Plasmid#178906Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseMutationCTG>AGT miR16-5p MRE1 mutatedPromoterSV40 promoterAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE153-mut4
Plasmid#178909Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseMutationTGC>CAT miR153-3p MRE mutatedPromoterSV40 promoterAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only