We narrowed to 11,028 results for: HA

1. T7RNAP Cyslight V174C

   Plasmid

   #205078

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Article

   Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

   Insert

   T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
   

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S V174C

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBlastR-12xLexAOp-mCherry-MiniWhite

   Plasmid

   #165917

   Purpose

   Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable LexAOp Response Vector
   

   Use

   Synthetic Biology

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-miR-29b-1~29a

   Plasmid

   #51376

   Depositor

   Narry Kim

   Article

   Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

   Insert

   pri-miR-29b-1~29a (MIR29B1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCGN-ELK-1 Ets mut

   Plasmid

   #27157

   Purpose

   For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   ELK1 (ELK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R62E/R65E/Y66D

   Available Since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pTW194b63

   Plasmid

   #136930

   Purpose

   Directed evolution of SpCas10

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   npuC-gIII(C)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINTO-N-FH

   Plasmid

   #65924

   Purpose

   Inducible expression of proteins fused to an N-terminal Flag-HA tag (FH)

   Depositor

   Roberto Bonasio

   Article

   Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

   Type

   Empty backbone

   Tags

   FLAG-HA

   Expression

   Mammalian

   Promoter

   CMV/TetO2 (T-REx)

   Available Since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Tag/WT M87

   Plasmid

   #89322

   Purpose

   expression of WT M87 spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-460 bp deleted

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO {ChETA}on-WPRE

   Plasmid

   #111386

   Purpose

   AAV expression of ChETA (hChR2 with E123T/H134R mutations) fused to HA tag driven by hSynapsin promoter in Cre-ON manner for optogenetic activation

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Insert

   ChETA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   humanized ChR2, E123T/H134R

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Tag/M1C448Y

   Plasmid

   #89319

   Purpose

   expression of M1 C448Y spastin in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Mutation

   1-194 bp of 5'UTR deleted, C448Y

   Promoter

   CMV

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBlastR-5xUAS-sfGFP-GMRWhite

    Plasmid

    #165907

    Purpose

    Blasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. P[acman]-Omega2

    Plasmid

    #165861

    Purpose

    Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-NES-MBP-cATOM(Ras)

    Plasmid

    #209711

    Purpose

    Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    NES-MBP-cATOM(Ras)
    

    Tags

    NES-MBP

    Expression

    Mammalian

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-MAPKAP1.1-FHH-IRES-Puro

    Plasmid

    #120705

    Purpose

    Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 Isoform 1
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. hRad51(A190L-A192L)–Cas9(D10A)

    Plasmid

    #125562

    Purpose

    Expresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51(A190L A192L)–Cas9(D10A) (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBlastR-5xUAS-sfGFP-MiniWhite

    Plasmid

    #165910

    Purpose

    Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Tag/M87 C448Y

    Plasmid

    #89320

    Purpose

    expression of M87 C448Y spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Expression

    Mammalian

    Mutation

    1-460 bp deleted

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRE/WT SP with 5'UTR

    Plasmid

    #89330

    Purpose

    Inducible expression of WT spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Expression

    Mammalian

    Promoter

    Tet-responsive P tight

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR'-ROMK1-CITE-GFP

    Plasmid

    #21322

    Depositor

    Jay Nadeau

    Article

    Nadeau et al J Neurophysiol. 2000 Aug . 84(2):1062-75

    Insert

    ROMK1 (Kcnj1 Rat)
    

    Use

    Lentiviral

    Tags

    GFP (bicistronic with CITE/IRES)

    Expression

    Mammalian

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pR6Kg-Alpha1spm

    Plasmid

    #165866

    Purpose

    Modified Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-FLAG-Lin28BdelN70

    Plasmid

    #51374

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    Lin28B (LIN28B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal 70 amino acids deletion

    Promoter

    CMV

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only