We narrowed to 150,834 results for: ins

1. pRXG

   Plasmid

   #113643

   Purpose

   This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.

   Depositor

   Jeffrey Barrick

   Article

   Monk et al ACS Synth Biol. 2016 Sep 20.

   Insert

   mRFP1-linker-sfGFP
   

   Use

   Synthetic Biology

   Available Since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200406

   Purpose

   Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   MCP-ADAR2dd(E488Q)-NES
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   E488Q (hyperactive)

   Promoter

   CMV

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-DeltaJunD

   Plasmid

   #68547

   Purpose

   AAV expression of DeltaJunD

   Depositor

   Eric Nestler

   Article

   Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

   Insert

   DeltaJunD-IRES-GFP
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   truncated splice variant

   Promoter

   CMV

   Available Since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pSEM253 - sgRNA mosTI hygroR

   Plasmid

   #159827

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI hygroR
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AICSDP-166:CDH1-mEGFP

   Plasmid

   #193921

   Purpose

   Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1

   Depositor

   Allen Institute for Cell Science

   Insert

   cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
   

   Use

   AAV and CRISPR; Donor template

   Tags

   linker-mEGFP

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Dec. 22, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pFA6a-link-ymTq2-URA3

   Plasmid

   #168057

   Purpose

   Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Tags

   ymTurquoise2

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEM270 - MCS mosTI hygroR target

   Plasmid

   #159826

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   MCS mosTI hygroR target
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEM321 - array co-injection fragment mosTI hygroR

   Plasmid

   #159828

   Purpose

   Single copy or array insertion by MosTI using split hygroR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   array co-injection fragment mosTI hygroR
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSETb-rsGreen0.7

   Plasmid

   #78176

   Purpose

   Expresses rsGreen0.7 in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreen0.7
   

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEM271 - MCS mosTI NeoR target

    Plasmid

    #159830

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI NeoR target
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEM254 - sgRNA mosTI neoR

    Plasmid

    #159831

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI neoR
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM322 - array co-injection fragment mosTI neoR

    Plasmid

    #159832

    Purpose

    Single copy or array insertion by MosTI using split NeoR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI neoR
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PKG I alpha WT

    Plasmid

    #16390

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-RT005-HaloGPM

    Plasmid

    #163385

    Purpose

    2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyCatcher002-oDi

    Plasmid

    #124661

    Purpose

    Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oDi
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AICSDP-80:UBTF-HaloTag

    Plasmid

    #133963

    Purpose

    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF

    Depositor

    Allen Institute for Cell Science

    Insert

    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    

    Use

    CRISPR

    Tags

    HaloTag-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVpuroATT

    Plasmid

    #47539

    Purpose

    Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

    Depositor

    David Weinstock

    Article

    Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pFUGW-hGtACR1-EYFP

    Plasmid

    #67795

    Purpose

    Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

    Depositor

    John Spudich

    Article

    Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

    Insert

    hGtACR1
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTK14

    Plasmid

    #37360

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    LGN (GPSM2 Human)
    

    Use

    Retroviral

    Tags

    GFP, S-tag, and TEV

    Expression

    Mammalian

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. IL-8 toehold switch sensor

    Plasmid

    #111908

    Purpose

    Toehold switch sensor to detect IL-8 mRNA with GFP output

    Depositor

    James Collins

    Article

    Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

    Insert

    IL8 toehold switch sensor
    

    Use

    Synthetic Biology

    Promoter

    T7

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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