We narrowed to 25,667 results for: promoter

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pcDNA5 FRT/TO Plk4 ∆24-mCherry

Plasmid

#80271

Purpose

mammalian expression plasmid for Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pHW530 (Prab-3::cGAL-N::let-858 3'UTR)

Plasmid

#107131

Purpose

cGAL-N driver under the control of C. elegans rab-3 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans rab-3 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-Archer1-EGFP

Plasmid

#60423

Purpose

Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

Depositor

Viviana Gradinaru

Article

Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

Insert

Archer1-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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AV15_pCAG.Cas9.gRNA.S1

Plasmid

#199259

Purpose

Expression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNA

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Inserts

SpCas9 nuclease
AAVS1-targeting gRNA

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG promoter and RNA polymerase III promoter for …

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-3xFlag-ETO

Plasmid

#12507

Depositor

Dong-Er Zhang

Article

Biggs et al Mol Cell Biol. 2006 Oct . 26(20):7420-9.

Insert

ETO (RUNX1T1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pWPI-E2F3

Plasmid

#114298

Purpose

Constitutive expression of the E2F3 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

E2F3 (E2F3 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2+ Gal4-GBP2-IRES-GBP7-p65

Plasmid

#50020

Purpose

Multi-purpose expression of a GFP-dependent transcription factor

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD

Use

Multi-purpose expression

Promoter

CMV-immediate early promoter and SP6 promoter

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pIRES-Puro-TCPTP

Plasmid

#33366

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Expression

Mammalian

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
Flag-Craf R89L

Plasmid

#118333

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf R89L (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

R89L

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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-2.5Kb MMP10

Plasmid

#53973

Purpose

Luciferase reporter for mouse MMP10 2.5kb promoter

Depositor

Michael Chin

Article

Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

Insert

mouse MMP10 2.5Kb promoter (Mmp10 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PCR from C57BL6/J

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-Turbo-NES-YFP-STOP-L2

Plasmid

#127358

Purpose

gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, NES, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Nipbl_Neo_FKBPF36V_mScarlet

Plasmid

#163883

Purpose

Acute Depletion NIPBL-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Nipbl (Nipbl Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pVRa14_3200

Plasmid

#49666

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF14_3200

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FBL6-FLAG3C

Plasmid

#19904

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

FBL6 (FBXL6 Human)

Tags

FLAG3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.3

Plasmid

#190797

Purpose

aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP

Expression

Insect

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-trc- YFP

Plasmid

#186569

Purpose

p15A ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Expression

Bacterial

Promoter

trc promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pWR76

Plasmid

#202776

Purpose

episomal fluorescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHR V5-RNF25 dRING_IRES-mCherry

Plasmid

#198387

Purpose

RNF25 (?RING) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RNF25 (RNF25 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

RING deletion (135-201)

Promoter

EF1A

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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