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pGL3-noSV40-humanEC1.45_p300peak1-2_del6

Plasmid

#173978

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #6

Use

Luciferase

Mutation

Tiled deletion #6

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del4

Plasmid

#173976

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #4

Use

Luciferase

Mutation

Tiled deletion #4

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del10

Plasmid

#173982

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #10

Use

Luciferase

Mutation

Tiled deletion #10

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del9

Plasmid

#173981

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #9

Use

Luciferase

Mutation

Tiled deletion #9

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del11

Plasmid

#173983

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #11

Use

Luciferase

Mutation

Tiled deletion #11

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del2

Plasmid

#173974

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #2

Use

Luciferase

Mutation

Tiled deletion #2

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del5

Plasmid

#173977

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #5

Use

Luciferase

Mutation

Tiled deletion #5

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del7

Plasmid

#173979

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #7

Use

Luciferase

Mutation

Tiled deletion #7

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_p300peak1-2_del8

Plasmid

#173980

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with tiled deletion #8

Use

Luciferase

Mutation

Tiled deletion #8

Promoter

SV40 promoter

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast HA-GPAT4-ΔNs5

Plasmid

#173169

Purpose

Retroviral vector to express sgRNA resistant HA tagged human GPAT4 with mutation in CHP1 binding site

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)

Use

Retroviral

Tags

HA

Mutation

Synonymous mutations at sgRNA sites, amino acids …

Promoter

MMLV

Available Since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pBtPIPLC_N168C His

Plasmid

#173809

Purpose

Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

Depositor

Anne Gershenson, Mary F Roberts

Article

He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

Insert

Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase

Tags

6XHis

Expression

Bacterial

Mutation

Asn168Cys for labeling

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p1.2-Zeo-hVKORC1

Plasmid

#162776

Purpose

Human vitamin K oxidoreductase expression

Depositor

Ivan Vorobiev

Article

Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

Insert

VKORC1 vitamin K epoxide reductase complex subunit 1 (VKORC1 Human)

Expression

Mammalian

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3_dPKA-CAT1N17-mVenus

Plasmid

#168497

Purpose

Mammalian expression of the N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTCFL5-donor

Plasmid

#113808

Purpose

CRISPR donor plasmid to tag human transcription factor TCFL5 with GFP

Depositor

Kevin White

Insert

TCFL5 homology arms flanking EGFP-IRES-Neo cassette (TCFL5 Human)

Use

CRISPR

Mutation

hg38:20:62842215:T>A

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-3xFLAG-NLS-TPP1-OB

Plasmid

#79060

Purpose

Retroviral expression of 3xFLAG tagged, nuclear localized wild-type TPP1-OB folding domain

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (TPP1 Human)

Use

Retroviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Wild-type TPP-OB folding domain

Promoter

CMV

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 STAT1

Plasmid

#67116

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

STAT1 (STAT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-Syn-NES-SomajRGECO1a

Plasmid

#232842

Purpose

AAV transfer plasmid for Syn-mediated expression of soma-targeted jRGECO1a

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomajRGECO1a

Use

AAV

Tags

nuclear export sequence, 6xHis, T7, Xpress tag

Expression

Mammalian

Promoter

Syn

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-ARF

Plasmid

#89123

Purpose

mammalian expression of p14-hARF with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Article

Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

Insert

p14-hARF (CDKN2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCIBN-LacI

Plasmid

#103814

Purpose

BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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ET8-GPIba-6His

Plasmid

#102878

Purpose

Wild type human platelet membrane protein GPIb alpha (1-290aa) with 6Histag at the C-terminus

Depositor

Timothy Springer

Article

Fu et al Nat Commun. 2017 Aug 23;8(1):324. doi: 1

Insert

Human platelet membrane protein GPIb alpha 1-290aa (GP1BA Human)

Tags

6xHis tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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