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V5-W279A-short-UBASH3A

Plasmid

#192086

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Tags

V5

Expression

Mammalian

Mutation

W279A

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDD397

Plasmid

#91822

Purpose

AID FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Auxin-inducible degron (AID)

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcnab2

Plasmid

#192795

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcnab2

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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delta Zfp521

Plasmid

#104190

Purpose

Expression of delta Zfp521 in mammalian cells

Depositor

Kathryn Hentges

Article

AlDallal et al Leuk Res. 2016 Jul;46:10-7. doi: 10.1016

Insert

Zfp521 (Zfp521 Mouse)

Expression

Mammalian

Mutation

deletion of final 6 Zn fingers

Promoter

CMV

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBS NFATC1

Plasmid

#19203

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

NFATC1 (NFATC1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pDest472 SHP-1 S591A

Plasmid

#21805

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

Serine 591 changed to Alanine

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
bTub-GFP.766A

Plasmid

#112695

Purpose

expressed GFP in testes, under bTub promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

bTub-GFP-SV40

Expression

Insect

Promoter

bTub

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJH26_pBad_AraC_Para-sfGFP.1TAG

Plasmid

#163721

Purpose

Arabinose-inducible expression of green fluorescent protein encoding one amber codon

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.1TAG

Expression

Bacterial

Mutation

gfp.N39TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH27_pBad_AraC_Para-sfGFP.2TAG

Plasmid

#163722

Purpose

Arabinose-inducible expression of green fluorescent protein encoding two amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp.2TAG

Expression

Bacterial

Mutation

gfp.N39TAG,Y151TAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH25_pBad_AraC_Para-sfGFP.0TAG

Plasmid

#163720

Purpose

Arabinose-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

araC gfp

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSuperRetro human Hic-5

Plasmid

#11188

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Insert

Hic-5 shRNA (TGFB1I1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCG-B-mSpyCatcher-C

Plasmid

#87374

Purpose

An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

mSpyCatcher

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-celA(SP)-B

Plasmid

#87371

Purpose

An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

CelA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCG-A-xynA(SP)-B

Plasmid

#87372

Purpose

An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

XynA signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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V180 pLP Triple-Flag SD - Acceptor

Plasmid

#11707

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

June 22, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pHu6-gRNA-NT1

Plasmid

#81203

Purpose

hU6 expression of gRNA NT1

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA NT1

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pME-Gal80

Plasmid

#51620

Purpose

vertebrate expression of full-length Gal80

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

Gal80 (GAL80 Budding Yeast)

Use

Vertebrate expression

Available Since

March 27, 2014

Availability

Academic Institutions and Nonprofits only

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pSG5-MAGE-A11

Plasmid

#89096

Purpose

mammalian expression of full-length human MAGE-A11

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Expression

Mammalian

Promoter

SV40

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLK15

Plasmid

#37253

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

Scribble (SCRIB Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 382-1630

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pMT-GFP-SAK

Plasmid

#31755

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sak (SAK Fly)

Tags

GFP

Expression

Insect

Mutation

A115S

Promoter

Metallothionein

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only