We narrowed to 166,096 results for: addgene

1. PPP2R2B B6.5 gRNA

   Plasmid

   #90848

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PPP2R2B

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PPP2R2B (Guide Designation B6.5)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX302 IL10RA-V5 puro

   Plasmid

   #47552

   Purpose

   Lentiviral expression of V5-tagged IL10RA in mammalian cells

   Depositor

   Kevin Janes

   Article

   Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

   Insert

   IL10RA (IL10RA Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

3. TET-pLKO.1 PURO shSMAD4 #2

   Plasmid

   #83091

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human SMAD4

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shSMAD4 (SMAD4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pFUW-TetO-DsRed-cNLS

   Plasmid

   #138533

   Purpose

   Lentiviral inducible expression of DsRed-Express2 with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   DsRed-Express2
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Promoter

   TRE-CMV

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-hSyn-Gαi*-BERKY3

   Plasmid

   #158428

   Purpose

   BRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSyn

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSLIK_hygro_3xflag_Luciferase

   Plasmid

   #136528

   Purpose

   Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   Luciferase
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   3x FLAG

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hmga2 3'UTR m2 luciferase (Luc-m2)

   Plasmid

   #14786

   Depositor

   David Bartel

   Article

   Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

   Insert

   Luc-m2 (Hmga2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   m2 mutant (see article and author's map for …

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-RIPK3[S227D]-FLAG

   Plasmid

   #199320

   Purpose

   mutated phosphorylation site (MLKL interaction permanent active)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 227 to Aspartic Acid

   Available Since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-RIPK3[S227A]-FLAG

   Plasmid

   #199318

   Purpose

   mutated phosphorylation site (MLKL interaction inhibited)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 227 to Alanine

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKPY197

    Plasmid

    #62599

    Purpose

    Encodes Thr412Gly-CePheRS Alpha Subunit

    Depositor

    David Tirrell

    Article

    Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

    Insert

    Thr412Gly-CePheRS Alpha Subunit
    

    Tags

    HIS

    Expression

    Worm

    Mutation

    Thr412Gly

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. RFN_EGFP_81

    Plasmid

    #60073

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

12. NAE1 G7.3 gRNA

    Plasmid

    #90776

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NAE1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NAE1 (Guide Designation G7.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEN_TT 3xFLAG-KIF20A

    Plasmid

    #192297

    Purpose

    Gateway entry vector for an inducible 3xFLAG-KIF20A

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    KIF20A (KIF20A Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MAP2-CD4

    Plasmid

    #43916

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human MAP2 promoter

    Available Since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

15. PLK1 G5.1 gRNA

    Plasmid

    #90834

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK1 (Guide Designation G5.1)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-PITCh-TUBB4B

    Plasmid

    #207785

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-hSyn-Gαq*-BERKY1

    Plasmid

    #158430

    Purpose

    BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. TAL2261

    Plasmid

    #36700

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BMPR1A-TALEN_Right (BMPR1A Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

19. TAL2260

    Plasmid

    #36699

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BMPR1A-TALEN_Left (BMPR1A Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. EGFP-Rab8b in pCSDEST2

    Plasmid

    #140881

    Purpose

    Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab8b (rab8b Zebrafish)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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