Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 14,321 results for: cas9

Showing: 5321 - 5340 of 14321 results

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pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

Plasmid

#211821

Purpose

PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Publication

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9-ABE8e, tracrRNA and Puromycin resistance gene

Expression

Mammalian

Promoter

chicken β-actin promoter, U6 promoter and hPGK pr…

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-FLAG-dCas9

Plasmid

#190130

Purpose

Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Publication

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

FLAG

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_A-AtUBI10-dCas9-24XGP41

Plasmid

#202011

Purpose

Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, Terminator: HSP

Depositor

Michael Smanski

Publication

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo level 1 vector

Promoter

AtUBI10

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSpCas9-(BB)-2A-Puro-HsHelz-sgRNA_AH

Plasmid

#148897

Purpose

Mammalian Expression of HsHelz-sgRNA

Depositor

Elisa Izaurralde

Publication

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz-sgRNA (HELZ Human)

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

Plasmid

#148848

Purpose

Mammalian Expression of HsAGO2-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA1 (AGO2 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

Plasmid

#148849

Purpose

Mammalian Expression of HsTNRC6A-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsTNRC6A-sgRNA1

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

Plasmid

#148850

Purpose

Mammalian Expression of HsTNRC6B-sgRNA1

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA1

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH

Plasmid

#148851

Purpose

Mammalian Expression of HsAGO2-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsAGO2-sgRNA2 (AGO2 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

Plasmid

#148852

Purpose

Mammalian Expression of HsTNRC6A-sgRNA2

Depositor

Elisa Izaurralde

Insert

HsTNRC6A-sgRNA2

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

Plasmid

#148853

Purpose

Mammalian Expression of HsTNRC6B-sgRNA3

Depositor

Elisa Izaurralde

Insert

HsTNRC6B-sgRNA3

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

Plasmid

#148854

Purpose

Mammalian Expression of HsAGO1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO1-sgRNA (AGO1 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

Plasmid

#148856

Purpose

Mammalian Expression of HsNot1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsNot1-sgRNA (CNOT1 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

Plasmid

#148857

Purpose

Mammalian Expression of HsPatL1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsPatL1-sgRNA

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

Plasmid

#148858

Purpose

Mammalian Expression of HsTNRc6C-sgRNA

Depositor

Elisa Izaurralde

Insert

HsTNRc6C-sgRNA

Use

CRISPR

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna2

Plasmid

#192796

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna2

Depositor

Marta Soden

Publication

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna2

Use

AAV and CRISPR

Promoter

U6

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna6

Plasmid

#192797

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna6

Depositor

Marta Soden

Publication

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna6

Use

AAV and CRISPR

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ECT2_sgRNA

Plasmid

#183875

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-H2BC11_sgRNA

Plasmid

#183881

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

H2BC11 sgRNA spacer (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-MYH10_sgRNA

Plasmid

#183886

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

MYH10 sgRNA spacer (MYH10 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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