We narrowed to 17,890 results for: eng
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Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRExpressionBacterial and YeastPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-PEmaxRH
Plasmid#206884PurposeExpresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEMax Delta RNAseH
TagsFLAGPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.12_GagZip-MCP-P2A-eGFP
Plasmid#205527PurposeExpress HIV GagZip-MCP-P2A-eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGagZip-MCP
ExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.18_PB-SDC4-PGK-Blast
Plasmid#205563PurposeExpress human SDC4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSDC4 (SDC4 Human)
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAM1.21_PB-UGCG-PGK-Blast
Plasmid#205566PurposeExpress human UGCGDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUGCG (UGCG Human)
UsePiggybacExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DIO-NS1
Plasmid#175273PurposeAAV vector mediating Cre-depenent expression of NS1 gene of YFV-17DDepositorInsertNonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
UseAAVPromoterSynapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NLS-HA-2xMCP-KRAB
Plasmid#126589PurposeExpresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backboneDepositorInsert2XMCP-KRAB
UseLentiviralTagsNLS-HAExpressionMammalianPromoterhuman ubiquitin C promoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
ESR1-luciferase
Plasmid#113351Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertESR1-enhancer (ESR1 Human)
UseLuciferaseAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4
Plasmid#98975PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA sgRNAs pair 4 and Cas9(D10A)
ExpressionMammalianAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
EKARdual
Plasmid#99870PurposeMeasure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrateDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEKAR
TagsShadowG and mTFP1ExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PA(WT)-Vav2
Plasmid#86977PurposePI(DM): Photo-activatable/light sensitiveDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsmCherryExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX394 Neisseria cinerea Cas9
Plasmid#68331PurposeNeisseria cinerea Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNcCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSC-IntE2
Plasmid#52265PurposeBacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linkerDepositorAvailable sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
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835 pcDNA3 HA PTEN G129R
Plasmid#10929DepositorAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
nuc5-
Bacterial strain#40804DepositorBacterial resistanceAmpicillinAvailable sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
EcNR2.dnaG.Q576A
Bacterial strain#41700DepositorPublicationBacterial resistanceAmpicillinAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMALcPP-MS2cp
Plasmid#203351PurposeExpresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteriaDepositorInsertMS2 coat protein
TagsHx6-MBPExpressionBacterialMutationV75E and A81G, to prevent capsid assemblyAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
MMLV-RT for Split-PE (pEH45)
Plasmid#190105PurposeExpresses MMLV-RT (full-length, from PE2) for Split-PE useDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLV-RT
TagsbpNLSExpressionMammalianMutationRT pentamutant from PE2 (D200N, T306K, W313F, T33…PromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo
Plasmid#167901PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only