We narrowed to 7,288 results for: Ank

1. p3xP3-EGFP.vas-int.NLS

   Plasmid

   #60948

   Purpose

   Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

   Depositor

   Frank Schnorrer

   Article

   Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

   Insert

   vas-phiC31 integrase.NLS
   

   Use

   Rmce

   Promoter

   vasa

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSKB-

   Plasmid

   #62540

   Purpose

   Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2196

   Plasmid

   #67549

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTXB1-CII

   Plasmid

   #51566

   Purpose

   Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

   Depositor

   Sankar Adhya, Francisco Malagon

   Insert

   cII
   

   Tags

   Intein-Chiting Binding Domanin

   Expression

   Bacterial

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTVGFP

   Plasmid

   #169445

   Purpose

   A vector with two different MCSs flanking an attP integration site and a floxable pax-GFP marker

   Depositor

   Jean-Paul Vincent

   Article

   Yu et al Dev Cell. 2020 Sep 14;54(5):583-592.e5.

   Type

   Empty backbone

   Use

   Targeting vector

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levels

   Plasmid

   #204801

   Purpose

   Bacterial expresison of Enterovirus D68 2A protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   D68 2A protease
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. MARS

   Plasmid

   #205232

   Purpose

   Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
   

   Tags

   Nuclear Export Sequence and mScarlet-i

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB2399

   Plasmid

   #67550

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pES133

   Plasmid

   #127552

   Purpose

   Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13

   Depositor

   Emmanuele Severi, Gavin Thomas

   Insert

   F5-flanked tetracycline resistance cassette
   

   Expression

   Bacterial

   Mutation

   Multiple SNPs in tetA (originally from pBR322) to…

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-STK32C_IDG-K

    Plasmid

    #170610

    Purpose

    Gateway destination clone of STK32C (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-STK32C (STK32C Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5

    Plasmid

    #61257

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pESR1-donor

    Plasmid

    #112337

    Purpose

    CRISPR donor plasmid to tag human transcription factor ESR1 with GFP

    Depositor

    Kevin White

    Insert

    ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
    

    Use

    CRISPR

    Mutation

    Unknown

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBetaActin-TUBB2a-mNeonGreen

    Plasmid

    #191323

    Purpose

    Expresses fluorescent labeled beta Tubulin to measure microtubule density

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    TUBB2a-mNeonGreen (Tubb2a Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFOXF2-donor

    Plasmid

    #113816

    Purpose

    CRISPR donor plasmid to tag human transcription factor FOXF2 with GFP

    Depositor

    Kevin White

    Insert

    FOXF2 homology arms flanking EGFP-IRES-Neo cassette (FOXF2 Human)
    

    Use

    CRISPR

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKG113

    Plasmid

    #63073

    Purpose

    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    CENP-T (CENPT Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    E. coli codon optimized. G217R mutation compared …

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

    Plasmid

    #61256

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-miniTurbo-V5-STK32A_IDG-K

    Plasmid

    #170608

    Purpose

    Gateway destination clone of STK32A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-STK32A (STK32A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pERF-donor

    Plasmid

    #112299

    Purpose

    CRISPR donor plasmid to tag human transcription factor ERF with GFP

    Depositor

    Kevin White

    Insert

    ERF homology arms flanking EGFP-IRES-Neo cassette (ERF Human)
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB2375

    Plasmid

    #67546

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked natMX marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pISL1-donor

    Plasmid

    #113820

    Purpose

    CRISPR donor plasmid to tag human transcription factor ISL1 with GFP

    Depositor

    Kevin White

    Insert

    ISL1 homology arms flanking EGFP-IRES-Neo cassette (ISL1 Human)
    

    Use

    CRISPR

    Mutation

    Unknown

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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