We narrowed to 28,734 results for: CRISPR

1. pCS2-3xFLAG-NLS-SpCas9-NLS

   Plasmid

   #51307

   Purpose

   Cas9 expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Insert

   3xFLAG-NLS-SpCas9-NLS
   

   Use

   CRISPR; Xenopus tropicalis expression

   Tags

   3x Flag and NLS

   Promoter

   CMV

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE TRE dCas9

   Plasmid

   #50915

   Purpose

   Tet-regulatable dCas9 lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ826

   Plasmid

   #39316

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Histidine 840 to Alanine (H840A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ825

   Plasmid

   #39315

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A)

   Promoter

   T7

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. SS9_RNA

   Plasmid

   #71656

   Purpose

   SS9-targeting gRNA for co-transformation with pSS9

   Depositor

   Ryan Gill

   Article

   Bassalo et al ACS Synth Biol. 2016 Apr 22.

   Insert

   SS9 gRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. gRNA_GFP-T2

   Plasmid

   #41820

   Purpose

   Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_GFP-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGH153_MS2-AIDΔ-Hygro

   Plasmid

   #85416

   Purpose

   lentiviral expression vector for MS2-AIDΔ fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AIDΔ and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCXLE-dCas9VP192-T2A-EGFP

   Plasmid

   #69536

   Purpose

   Episomal plasmid encoding dCas9VP192

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   dCas9-dCas9VP192-EGFP
   

   Use

   CRISPR

   Tags

   EGFP

   Promoter

   CAG

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-T3-hCAS-pA

    Plasmid

    #48625

    Purpose

    Expresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii, Kunihiko Naito

    Article

    Fujii et al Nucleic Acids Res. 2013 Aug 30.

    Insert

    Codon optimized Cas9
    

    Use

    CRISPR

    Tags

    FLAG, NLS, and polyA

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry

    Plasmid

    #84248

    Purpose

    Expresses Sp sgTRE3G gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgTRE3G
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. M-NMn-VP64

    Plasmid

    #48676

    Purpose

    Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null

    Promoter

    CMV

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6a:sgRNA(tyr)

    Plasmid

    #64250

    Purpose

    expresses sgRNA(tyr) under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Insert

    U6a:sgRNA (tyr)
    

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. M-ST1-sgRNA

    Plasmid

    #48672

    Purpose

    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    

    Use

    CRISPR

    Promoter

    hU

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGH183_MS2-AIDΔDead-Hygro

    Plasmid

    #85414

    Purpose

    lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AIDΔDead and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. piCRg Entry

    Plasmid

    #58904

    Purpose

    Cas9/gRNA Entry expression plasmid

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    hSpCas9
    

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. USP19 sgRNA1

    Plasmid

    #78585

    Purpose

    delete USP19 gene in human cell

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    USP19 sgRNA1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

18. M-SPn-VP64

    Plasmid

    #48674

    Purpose

    Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Mutation

    nuclease-null

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pGH156_MS2-AID-Hygro

    Plasmid

    #85415

    Purpose

    lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    MS2-AID and Hygromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

    Plasmid

    #84249

    Purpose

    Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgSV40
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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