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pMT-P2

Plasmid

#120864

Purpose

Encodes permuteposon P2 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P2

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMT-P3

Plasmid

#120865

Purpose

Encodes permuteposon P3 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P3

Use

Synthetic Biology

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDH22

Plasmid

#83798

Purpose

Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKM343

Plasmid

#71487

Purpose

Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Insert

loxP-hyg-cat-loxP

Use

Source of loxp-hyg-cat- loxp for pcr amplificatio…

Expression

Bacterial

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2313

Plasmid

#137053

Purpose

Endogenous Locus DiCre Expression Cassette

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Type

Empty backbone

Use

Donor vector for endogenous locus dicre

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBS4

Plasmid

#83793

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Tags

CFP

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pUBN-paGFP-Dest

Plasmid

#105108

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Tags

paGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaGSCYP101A1

Plasmid

#108206

Purpose

CYP101A1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

CYP101A1

Expression

Bacterial

Promoter

lac promoter

Available Since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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FRP1483

Plasmid

#64511

Purpose

Expresses destabilized Ametrine in yeast.

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

Ametrine-ADH1-tail

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMIG-RhoC

Plasmid

#14119

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

Human RhoC (RHOC Human)

Use

Retroviral

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMT-P1

Plasmid

#120863

Purpose

Encodes permuteposon P1 for MuA transposase-mediated circular permutation

Depositor

Jonathan Silberg

Article

Atkinson et al Methods Enzymol. 2019;621:191-212. doi:

Insert

Permuteposon P1

Use

Synthetic Biology

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFS119

Plasmid

#12379

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

ade6, thymidine kinase

Expression

Yeast

Available Since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-SznE

Plasmid

#139282

Purpose

expresses SznE in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

sznE

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBM5218

Plasmid

#18796

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Ty5-HIS3AI

Expression

Yeast

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pRS415-recR

Plasmid

#49457

Purpose

single copy recombinase yeast expression plasmid

Depositor

Marc Gartenberg

Article

Ansari et al Methods. 1999 Feb;17(2):104-11.

Insert

recR

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pBES2

Plasmid

#83800

Purpose

Useful for tagging yeast proteins with N-terminal Venus tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

Venus

Expression

Yeast

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFS118

Plasmid

#12378

Depositor

Nick Rhind

Article

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

ura4, thymidine kinase

Expression

Yeast

Available Since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pGL2316

Plasmid

#137063

Purpose

loxP (empty) expression cassette: n-terminal GFP

Depositor

Jeremy Mottram

Article

Duncan et al Methods Mol Biol. 2019;1971:211-224. doi

Type

Empty backbone

Use

Leishmania floxed gene expression

Tags

GFP

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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rpaA-

Plasmid

#101847

Purpose

deletes rpaA

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

gentamicin resistance casette replacing rpaA

Use

Cloning vector

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-MAP4-C-10

Plasmid

#57399

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

MAP4

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits