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pTGMP-p16/p19.478

Plasmid

#32717

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

p16/p19

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
Flag-FOXN4

Plasmid

#153140

Purpose

Expression of Flag tagged human FOXN4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXN4 (FOXN4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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p5xFbx15 (-544/-527)-TK-Luc

Plasmid

#15686

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

5 x Fbx15 enhancer (Oct/Sox binding site)

Use

Luciferase

Mutation

Wild-type

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pLenti_CMV:Flag-CeNLuc_SV40:PuroR

Plasmid

#183036

Purpose

Expresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

CeNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCol-TGM-luc.1309

Plasmid

#32720

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

luciferase

Use

Mouse Targeting and RNAi

Promoter

TRE and Tre

Available Since

March 2, 2012

Availability

Academic Institutions and Nonprofits only
nGR2175-gs10-NLS-VPR

Plasmid

#188264

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nGR2175

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MKRN1_H307E

Plasmid

#78756

Purpose

To overexpress MKRN1_H307E in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H307E

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3

Plasmid

#171497

Purpose

T7 promotor drives in vitro transcription of mClover3 mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

mClover3

Use

Pgehme

Promoter

T7

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiR_Luc-A

Plasmid

#25765

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA with GFP coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Tags

GFP

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GIN-recipient

Plasmid

#11656

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP-IRES-Neo expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP, IRES, and Neo

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pMpGWB207

Plasmid

#68598

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pAct5C-Hh

Plasmid

#37382

Depositor

Norbert Perrimon

Article

Nybakken et al Nat Genet. 2005 Dec;37(12):1323-32. Epub

Insert

hedgehog (hh Fly)

Expression

Insect

Promoter

actin5C

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-Amida

Plasmid

#15362

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
A3D-Cas9n-UGI

Plasmid

#119137

Purpose

Base editor made from APOBEC3D

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

APOBEC3D-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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NavB4-GFP/pEGFP-N1

Plasmid

#197310

Purpose

Plasmid expressing an antigen targeting the Nav beta4 sodium channel subunit

Depositor

Lori Isom

Article

Aman et al J Neurosci. 2009 Feb 18;29(7):2027-42. d

Insert

NavB4-GFP (Scn4b Rat)

Tags

eGFP

Expression

Mammalian

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTH652-RLuc/maxFLuc

Plasmid

#29698

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

Firefly Luciferase

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pMpGWB212

Plasmid

#68603

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSR47

Plasmid

#69258

Purpose

pmyo-3::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

myo-3

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTRCHisB-TRF2∆B

Plasmid

#53208

Purpose

Expressed N-terminally Hexahistine tagged TRF2 Delta Basic protein

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 Delta Basic (TERF2 Human)

Tags

6x His

Expression

Bacterial

Mutation

contains amino acids 47-500

Promoter

Trc

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

Plasmid

#183803

Purpose

AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

E2-Crimson-NLS

Use

AAV

Tags

NLS

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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