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Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon opti…PromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(C162F)-3F-BirA
Plasmid#220149PurposeExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged cysteine 162 to phenylalanine; codon opti…PromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet2 CD
Plasmid#72219PurposeExpress flag-tagged mouse Tet2 catalytic domain.DepositorInsertTen-Eleven Translocation 2 (Tet2 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET2 catalytic domain.PromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet2 CD
Plasmid#72219PurposeExpress flag-tagged mouse Tet2 catalytic domain.DepositorInsertTen-Eleven Translocation 2 (Tet2 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET2 catalytic domain.PromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 K720A
Plasmid#19939DepositorInsertcullin 1 K720A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a NEDD8 modification mutantPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 K720A
Plasmid#19939DepositorInsertcullin 1 K720A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationCUL1 with a NEDD8 modification mutantPromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL2-pCMV7.1
Plasmid#155020PurposeN-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vectorDepositorInsertCullin 2 (CUL2 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL2-pCMV7.1
Plasmid#155020PurposeN-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vectorDepositorInsertCullin 2 (CUL2 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL5-pCMV7.1
Plasmid#155024PurposeN-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vectorDepositorInsertCullin 5 (CUL5 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL5-pCMV7.1
Plasmid#155024PurposeN-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vectorDepositorInsertCullin 5 (CUL5 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-mAirn-R-loop
Plasmid#134900PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInsertmAirn promoter (Airn Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-mAirn-R-loop
Plasmid#134900PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInsertmAirn promoter (Airn Mouse)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hSNRPN-R-loop
Plasmid#134902PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInserthSNRPN promoter (SNRPN Human)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-hSNRPN-R-loop
Plasmid#134902PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorInserthSNRPN promoter (SNRPN Human)
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTag-BFP-C-h-Rab5a-c-Myc
Plasmid#79801PurposeExpress TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).DepositorInsertRab5a (RAB5A Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTag-BFP-C-h-Rab5a-c-Myc
Plasmid#79801PurposeExpress TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).DepositorInsertRab5a (RAB5A Human)
UseTagsTagBFP and mycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX307-VHL(Y98H)-3F-BirA
Plasmid#220148PurposeExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged tyrosine 98 to histidine; codon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX307-VHL(Y98H)-3F-BirA
Plasmid#220148PurposeExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUBDepositorInsertvon Hippel-Lindau tumor suppressor (VHL Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationchanged tyrosine 98 to histidine; codon optimizedPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only