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pT2/shCol1a1/Scarlet_Seq1.2

Plasmid

#201404

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet-Seq1.1

Plasmid

#201401

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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HH13 Clone 13 heavy chain

Plasmid

#192177

Purpose

Clone 13 heavy chain (HH013)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13 heavy chain (HH013)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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HH13A Clone 13A heavy chain

Plasmid

#192178

Purpose

Clone 13A heavy chain (HH13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A heavy chain (HH13A)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1

Plasmid

#192470

Purpose

Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

Plasmid

#192469

Purpose

Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEThT-RvCAHS8

Plasmid

#192467

Purpose

Bacterial expression of RvCAHS8

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEThT-RvCAHS3

Plasmid

#192466

Purpose

Bacterial expression of RvCAHS3

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS12-min-GS

Plasmid

#192465

Purpose

Expression of RvCAHS12-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12-min

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-RvCAHS3-min-GS

Plasmid

#192463

Purpose

Expression of RvCAHS3-min-GS_linker-AcGFP1 in mammalian cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3-min

Tags

AcGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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HK13A Clone 13A light chain

Plasmid

#192182

Purpose

Clone 13A light chain (HK13A)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 13A light chain (HK13A)

Expression

Mammalian

Mutation

NA

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_139

Plasmid

#180565

Purpose

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-IAP-del-gRNA1

Plasmid

#185002

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

IAP-del-gRNA1 (Akp3 Mouse)

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_095

Plasmid

#180524

Purpose

dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168243

Purpose

"Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-DN

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

Plasmid

#168244

Purpose

"Rescue Rac-CA expression in neutrophil specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

Rac(guide resistant)-CA

Use

Zebrafish expression

Tags

mcherry

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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