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Showing: 1041 - 1080 of 2354 results

  3. dFP

    Plasmid
    #120542
    Purpose
    Expresses dFP biliverdin-binding fluorescence-activating full length protein in bacteria.
    Depositor
    Brian Chow
    Article
    Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/
    Insert
    de novo fluorescence-activating protein (dFP)
    Use
    Tags
    6xHis - TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    120542-plasmid-map-sequence-id-254195

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  6. p140Tol2-Olactb:SABER

    Plasmid
    #226829
    Purpose
    Expression of beta actin driven BFP and molecular barcode array for SABER-seq in zebrafish. Also contains heat-shock activated mCherry reporter and CreERT2.
    Depositor
    Bushra Raj
    Article
    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi
    Inserts
    BFP-SABER
    mCherry-t2A-CreERT2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Olactb and hsp70
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    226829-plasmid-map-sequence-id-455504

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  16. LIMK2 (LIMK2A-c052)

    Plasmid
    #110264
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    LIMK2 (LIMK2 Human)
    Use
    Tags
    His-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110264-plasmid-map-sequence-id-212269

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  21. pCas

    Plasmid
    #62225
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Depositor
    Sheng Yang
    Article
    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii
    Has Service
    Cloning Grade DNA
    Insert
    cas9
    Use
    CRISPR; Lambda red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native cas9 promoter
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    62225-plasmid-map-sequence-id-275267

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  27. Mito-SNAP

    Plasmid
    #206480
    Purpose
    Encodes a mitochondrially-targeted SNAP tag for mammalian cell expression
    Depositor
    Erika Holzbaur
    Article
    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7
    Insert
    Mitochondrial targeting signal from COX8A fused to SNAP (derived from human MGMT)
    Use
    Tags
    SNAPf
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    206480-plasmid-map-sequence-id-435222

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  33. Galt1-Grx1-roGFP1-iE

    Plasmid
    #230029
    Purpose
    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.
    Depositor
    Pablo Rivera-Fuentes
    Insert
    Galt1-Grx1-roGFP1-iE (B4GALT1 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230029-plasmid-map-sequence-id-458346

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  34. pYPQ131-tRNA2.0

    Plasmid
    #158393
    Purpose
    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Without promoter
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158393-plasmid-map-sequence-id-330365

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  37. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

    Plasmid
    #227775
    Purpose
    Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.
    Depositor
    Bushra Raj
    Article
    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi
    Inserts
    BFP-MCS
    mCherry-t2A-CreERT2
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Olactb and hsp70l
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    227775-plasmid-map-sequence-id-455547

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  39. FRB_SCF2_LD6_CTEV

    Plasmid
    #58879
    Purpose
    MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58879-plasmid-map-sequence-id-94240

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  40. FKBP_SCF2_LD6_NTEV

    Plasmid
    #58878
    Purpose
    MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58878-plasmid-map-sequence-id-94239

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Showing: 1041 - 1080 of 2354 results