We narrowed to 8,920 results for: Pol

1. pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA

   Plasmid

   #218869

   Purpose

   Mammalian expression of improved GABA sensor (negative change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2n
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S99A F104H R168P

   Promoter

   CAG

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 8xGliBS-IVS2-mCherry-NLS-polyA-Tol2

   Plasmid

   #84604

   Purpose

   Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

   Depositor

   James Chen

   Article

   Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

   Insert

   mCherry
   

   Use

   Zebrafish transgenesis

   Tags

   Expression

   Mutation

   Promoter

   8xGliBS-delta-crystallin minimal promoter

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

   Plasmid

   #165450

   Purpose

   Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

   Depositor

   Elvir Becirovic

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Inserts

   dCas9N-IntN
   3x Opn1mw promoter-targeting sgRNAs
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and short human rhodopsin promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-RHO-IntC-dCas9C-VPR-polyA

   Plasmid

   #166692

   Purpose

   Express C-terminal part of split dCas9-VPR

   Depositor

   Stylianos Michalakis

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Insert

   IntC-dCas9C-VPR
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   short human rhodopsin promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

   Plasmid

   #168291

   Purpose

   optimized miniCMV (OminiCMV)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   miniCMV

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-TgKI-MCS-p2A-tdTomato-polyA

   Plasmid

   #174027

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   p2A-tdTomato
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CAG-CasRX-P2A-EGFP-BGH PolyA

   Plasmid

   #154004

   Purpose

   Express CasRx

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   CasRx
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-bpNLS-hPol eta-2A-tagBFP-bGHpA

   Plasmid

   #193968

   Purpose

   Encodes a wildtype human Pol? (eta) with P2A-tagBFP marker driven by CAG promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   hPol eta (POLH Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

   Plasmid

   #174025

   Purpose

   This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

   Depositor

   Michel Bagnat

   Article

   Levic et al Development. 2021 Sep 8. pii: 272090. do

   Insert

   eGFP
   

   Use

   Zebrafish knock-in tagging

   Tags

   Expression

   Mutation

   Y40N

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-CMV-EGFP-PolB-PAMmut-Hygro

    Plasmid

    #176086

    Purpose

    EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by PolBKOg1

    Promoter

    CMV

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Polymerase expression - pCMV-Phi29(+exo) (LM2759)

    Plasmid

    #208964

    Purpose

    Unfused Phi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Phi29(+exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19-TgKI-MCS-mScarlet-MCS-polyA

    Plasmid

    #174023

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    mScarlet
    

    Use

    Zebrafish knock-in tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA

    Plasmid

    #89460

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Use

    Tags

    hinge + HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid

    #208969

    Purpose

    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    EcKlenow-BPNLS-CC(N5)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. single polypeptide FPX biosensor for both caspase-3 and -8

    Plasmid

    #60886

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-3 and 8
    

    Use

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLVX-Flag-Polß(D256A)-IRES-puro

    Plasmid

    #128677

    Purpose

    mammalian expression of Flag-Polß(D256A) protein with puromycin selection

    Depositor

    Robert Sobol

    Article

    Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D256A

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-EGFP barcode-10-SV40 polyA

    Plasmid

    #190874

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode10
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. RNA polymerase beta prime subunit FLAG

    Plasmid

    #102337

    Purpose

    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.

    Depositor

    Erin O'Shea

    Article

    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1

    Insert

    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    

    Use

    Tags

    3x FLAG with 3X GSGS linker

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

    Plasmid

    #155194

    Purpose

    Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated sequence of THBS1 (expresses Amino Acid…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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