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We narrowed to 1,088 results for: control gRNA

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Showing: 521 - 540 of 1088 results

  1. H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41)

    Plasmid
    #246055
    Purpose
    Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before D41) linked through a P2A sequence, under the same pol-III H1 promoter
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-D41
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246055-plasmid-map-sequence-id-495341

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  2. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    U6 and synthetic Probasin ARRx2
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68357-plasmid-map-sequence-id-122833

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  5. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid
    #241026
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven empty sgRNA
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241026-plasmid-map-sequence-id-482053

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  6. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  7. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177360
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Promoter
    Synapsine promoter and U6 promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177360-plasmid-map-sequence-id-353669

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  8. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177365
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression
    Depositor
    Naoki Yamamoto
    Article
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Promoter
    CMV and U6 promoter
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177365-plasmid-map-sequence-id-353664

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  11. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  13. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid
    #221002
    Purpose
    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    dCasRx-RBM25-IRES-BleoR
    Use
    CRISPR
    Tags
    NLS and NLS-HA
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    221002-plasmid-map-sequence-id-436162

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  14. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  15. pDual_dsCas9_Puro_hs_NC_chr1

    Plasmid
    #214688
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Mutation
    Puromycin resistance cassette has silent mutation…
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214688-plasmid-map-sequence-id-428817

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  16. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118157
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118157-plasmid-map-sequence-id-240364

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  19. GB4070

    Plasmid
    #187811
    Purpose
    Module for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).
    Depositor
    Diego Orzaez
    Article
    Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.
    Insert
    copper-inducible dCasEV2.1
    Expression
    Plant
    Available Since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    187811-plasmid-map-sequence-id-378054

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Showing: 521 - 540 of 1088 results