We narrowed to 2,916 results for: human cdnas
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Plasmid#49069PurposeExpresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC563S,C568S,C577S,C582S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)
Plasmid#49080PurposeExpresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC791S, C820S, C1052S in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)
Plasmid#49070PurposeExpresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationECL4 deletion of amino acids 562-582 in hNKCC1 in…PromoterCMVAvailable sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S,C724V (NT501)
Plasmid#49073PurposeExpresses human NKCC1 C723S and C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
UseTags3x Flag and mVenusExpressionMammalianMutationC723S,C724V in hNKCC1 in NT17PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-RecQL4
Plasmid#211399Purposemammalian expression of human RecQL4DepositorInsertRecQL4 (RECQL4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ULK2
Plasmid#97075PurposeExpresses ULK2 in mammalian cellsDepositorInsertULK2 (ULK2 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-PAFAH1B1
Plasmid#92430Purposemammalian expression of PAFAH1B1DepositorInsertPAFAH1B1 (PAFAH1B1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3myc-PR-LBD
Plasmid#89113Purposemammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tagDepositorInserthuman progesterone receptor ligand binding domain (PGR Human)
UseTagsmycExpressionMammalianMutationcontains Ligand Binding Domain (632-933)PromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngPDm
Plasmid#64040PurposeMammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertPax8 paired domain (Pax8 Mouse)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationpaired domain, aa 1-147, with aa 30 to 32 changed…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EngHDm
Plasmid#64038PurposeMammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)DepositorInsertTTF-1 (TTF1 dog)
UseTagsEng (N-terminal repressor domain of the drosophi…ExpressionMammalianMutationhomeodomain, aa 154-226, with aa 48–50 replaced b…PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1
Plasmid#114022PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell linesDepositorInserteGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-S2215Y
Plasmid#26037DepositorInsertmammalian target of rapamycin-S2215Y (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationS2215YPromoterAvailable sinceSept. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-R2505P
Plasmid#26038DepositorInsertmammalian target of rapamycin-R2505P (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationR2505PPromoterAvailable sinceSept. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1
Plasmid#114027PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell linesDepositorInserteGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1(+) eGFP p6 K27R
Plasmid#78583PurposeExpresses GFP p6 with K27R mutation in mammalian cellsDepositorInserteGFP p6
UseTagsExpressionMammalianMutationHIV-1 HXB2 p6 with conservative mutations (G78A, …PromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-62
Plasmid#208647PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro-sgRPL3-56
Plasmid#208979PurposepcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.DepositorInsertsgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo largeTcDNA
Plasmid#1780PurposeRetroviral expression of Large T Antigen. Used to create immortalized cell lines.DepositorInsertlarge T antigen (SV40gp6 simian virus)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
N370S GBA pcDNA3.1
Plasmid#188583PurposeEctopic expression N370S GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationN370SPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
L444P GBA pcDNA3.1
Plasmid#188582PurposeEctopic expression L444P GBADepositorInsertGBA (GBA1 Human)
UseTagsExpressionMammalianMutationL444PPromoterCMVAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only