Addgene

We narrowed to 834 results for: nanobody

Showing: 521 - 540 of 834 results

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCASCADE-LCAmp-trxB

Plasmid

#202465

Purpose

Expresses a guide RNA to silence trxB after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

trxB guide RNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

ugpB

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(1-157)

Plasmid

#220743

Purpose

GAL4-sfGFP(1-157) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(1-157)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(158-238)

Plasmid

#220744

Purpose

GAL4-sfGFP(158-238) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(158-238)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-NIR-FbLAG30

Plasmid

#220746

Purpose

GAL4-NIR-FbLAG30 construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNIR-FbLAG16

Plasmid

#220739

Purpose

NIR-FbLAG16 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG16

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNIR-FbLAG30

Plasmid

#220740

Purpose

NIR-FbLAG30 expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pNIR-Fb(718)

Plasmid

#220742

Purpose

NIR-Fb(718) expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-Fb(718)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pmEos2-N1 GBP (GBP-mEos2)

Plasmid

#162876

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein tagged with mEos2

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A8_His

Plasmid

#201718

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A8 (S lama glama (llama))

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_A10-5_His

Plasmid

#201720

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH_A10-5 (S lama glama (llama))

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOPINVHH_B5-5_His

Plasmid

#201721

Purpose

Expression of nanobody

Depositor

Ray Owens

Article

Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

Insert

VHH B5-5 (S lama glama (llama))

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP670nano3

Plasmid

#184666

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3-human alpha-tubulin

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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NbAlfa-GFP2

Plasmid

#201485

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbAlfa-GFP2

Use

Tags

GFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP2-NbALFA

Plasmid

#201486

Purpose

BRET2 detection of ALFA-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

GFP2-NbALFA

Use

Tags

GFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4 R105A

Plasmid

#196072

Purpose

Express mutant (R105A) SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4 R105A

Use

Tags

3XFLAG and 6XHis

Expression

Bacterial

Mutation

R105A mutation in SG4

Promoter

T7

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Use

Tags

His14 and bdNEDD8

Expression

Bacterial

Mutation

Promoter

Available since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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NbV5-eGFP

Plasmid

#201473

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-eGFP

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-vhhGFP4-hIgG1-Fc

Plasmid

#105576

Purpose

T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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NbV5-HALOTAG

Plasmid

#201482

Purpose

Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-HALOTAG

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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