We narrowed to 9,180 results for: mel

1. pFUGW-RapACR-EYFP

   Plasmid

   #111075

   Purpose

   Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR
   

   Use

   Lentiviral

   Tags

   EYFP

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM0641

   Plasmid

   #129006

   Purpose

   Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'-KanR-ColE1
   

   Expression

   Yeast

   Available Since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ1208

   Plasmid

   #44490

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
   

   Use

   Gateway expression clone

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUGW-RapACR_T111C-EYFP

   Plasmid

   #119195

   Purpose

   Fast anion channelrhodopsin mutant RapACR_T111C for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C
   

   Use

   Lentiviral

   Tags

   EYFP

   Mutation

   changed threonine 111 to cysteine

   Promoter

   Ubiquitin

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. All-in-one split-ADAR2

   Plasmid

   #170136

   Purpose

   AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. K401-iLid

   Plasmid

   #122484

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Matt Thomson

   Article

   Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

   Insert

   Kinesin 1-401 iLid His tag
   

   Tags

   His6 and iLid

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-401

   Promoter

   T7

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. K20‐scFv‐pSMBP2

   Plasmid

   #159940

   Purpose

   To generate baculovirus for insect cell expression of anti beta-1 integrin scFv K20

   Depositor

   Sandra Schmid

   Article

   Lakoduk et al Traffic. 2020 Sep;21(9):590-602. doi: 10

   Insert

   anti beta-1 integrin scFv
   

   Tags

   6xHis tag, FLAG tag, MBP fusion, Sortase A recogn…

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1(-) + SLNCR1

   Plasmid

   #86816

   Purpose

   mammalian expression of lncRNA LINC00673/SLNCR1

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. BirA*-HA-NES

   Plasmid

   #214473

   Purpose

   Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

   Depositor

   Natalie Ahn

   Article

   Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

   Insert

   BirA(R118G)
   

   Use

   Lentiviral; Gateway

   Tags

   HA tag and NES (Nuclear Exclusion Sequence)

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   EF-1a

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. XE49 pt Beta-catenin-myc

    Plasmid

    #16840

    Depositor

    Randall Moon

    Article

    Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

    Insert

    pt beta-catenin-myc (ctnnb1.L Frog)
    

    Use

    Xenopus expression

    Tags

    6x myc

    Mutation

    four point mutations introduced into the GSK-3 ph…

    Available Since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pGG184

    Plasmid

    #165604

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    'CAGCG' PAM replacing the 'CGGCG&#…

    Promoter

    lac

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-PIWI

    Plasmid

    #11591

    Depositor

    Edward Chan

    Article

    Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

    Insert

    Ago2 (AGO2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contain only aa482-869

    Available Since

    June 30, 2006

    Availability

    Academic Institutions and Nonprofits only

13. BoxB-MS2 adRNA (RAB7A-ACG site)

    Plasmid

    #170155

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(ACG)-MS2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

    Plasmid

    #63170

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-OLLAS"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MS2-adRNA (5'UAG)

    Plasmid

    #170126

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'UAG)-MS2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 and mouse U6

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL1180-HR-PUbEGFP-NLS

    Plasmid

    #176681

    Purpose

    Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    EGFP
    

    Expression

    Insect

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. BoxB-MS2 adRNA (RAB7A-UAG site)

    Plasmid

    #170138

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(UAG)-MS2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459-sg-tdTomato90/816

    Plasmid

    #179918

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159672

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    βTub-NLS-hSpCas9-T2A-GFP
    

    Tags

    T2A-GFP

    Expression

    Insect

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCETN1-IRFP670

    Plasmid

    #99879

    Purpose

    Expression of CETN1-iRFP670 in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    CETN1 (CETN1 Human)
    

    Tags

    iRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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