We narrowed to 15,989 results for: LEA

1. pET21-pelB-LaG-14

   Plasmid

   #172745

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-14
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-Flag-NEDD4L∆WW4

   Plasmid

   #166710

   Purpose

   expresses NEDD4L with inactivating mutation in fourth WW domain in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L W576A
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 576 to alanine

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-PP-NEDD4L-WW1(193-226)

   Plasmid

   #166701

   Purpose

   expresses NEDD4L WW1 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW1 domain
   

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-PP-NEDD4L-WW2(385-418)

   Plasmid

   #166702

   Purpose

   expresses NEDD4L WW2 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW2 domain
   

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-PP-NEDD4L-WW3(497-530)

   Plasmid

   #166703

   Purpose

   expresses NEDD4L WW3 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW3 domain
   

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-PP-NEDD4L-WW4(548-581)

   Plasmid

   #166704

   Purpose

   expresses NEDD4L WW4 in bacterial cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L WW4 domain
   

   Tags

   GST, HRV 3C site, and Thrombin site

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-Flag-NEDD4L∆WW1

   Plasmid

   #166707

   Purpose

   expresses NEDD4L with inactivating mutation in first WW domain in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

   Insert

   NEDD4L W221A
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed tryptophan 221 to alanine

   Promoter

   CMV promoter

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. IBMc180

   Plasmid

   #161942

   Purpose

   Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
   

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. IBMc226

    Plasmid

    #161944

    Purpose

    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

    Plasmid

    #149350

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMKO.1-shSpc25

    Plasmid

    #160960

    Purpose

    Spc25 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Spc25 shRNA (Spc25 Mouse)
    

    Use

    RNAi and Retroviral

    Promoter

    U6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPS1-Venus

    Plasmid

    #133118

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pYPS1-YFP
    

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMK2-Venus

    Plasmid

    #133119

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pCMK2-YFP
    

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGYP7-Venus

    Plasmid

    #133120

    Purpose

    Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    pGYP7-YFP
    

    Expression

    Bacterial and Yeast

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15-His-∆NGlpG(Y205F)

    Plasmid

    #155132

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y205F, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFloat.assemble(AB).LC_v1

    Plasmid

    #133083

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. Low copy. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFloat.assemble(AB).HC_v1

    Plasmid

    #133084

    Purpose

    Tier 1 destination vector for assembling gene cassettes intended for expression in bacteria. AB sites. High copy. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Destination vector

    Expression

    Bacterial

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPOR1CB0015

    Plasmid

    #117551

    Purpose

    Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
    

    Expression

    Plant

    Promoter

    2x35S+5'UTR OMEGA (pICH51288)

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPOR2KN0078

    Plasmid

    #117645

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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