We narrowed to 7,309 results for: ski

1. AT1G66830 X005_pECIA14

   Plasmid

   #114810

   Purpose

   Prey vector AT1G66830 X005_pECIA14 should be used with bait vector AT1G66830 X005_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G66830 (AT1G66830 Mustard Weed)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmiR-report-MYB-WT

   Plasmid

   #141114

   Purpose

   Reporter plasmid of WT MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   CMYB (MYB Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106179

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   hSynapsin

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP Kind2 S159/181/666A

   Plasmid

   #105310

   Purpose

   To study altered downstream functions of phospholylation-site null kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666A

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

   Plasmid

   #157699

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. PB-PE NG

   Plasmid

   #174554

   Purpose

   All-in-one prime editor piggyBac transposon, NG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

   Promoter

   CAG

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Blnk-SH2

   Plasmid

   #46408

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Blnk (BLNK Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 333-456

   Promoter

   Tac

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SIN40C.SFFV.mArid3a.IRES.GFP

   Plasmid

   #169309

   Purpose

   Constitutive overexpression of murine Arid3a cDNA with GFP as reporter fluorescent protein

   Depositor

   Jan-Henning Klusmann

   Article

   Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

   Insert

   Arid3a (Arid3a Mouse)
   

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmiR-report-MYB-mut

   Plasmid

   #141115

   Purpose

   Reporter plasmid of mutant MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   MYB (MYB Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   A to T mutations at m6A sites

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX Grap2-SH2

    Plasmid

    #46435

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grap2 (GRAP2 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 52-159

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCIBN-tagBFP-hNCL

    Plasmid

    #103805

    Purpose

    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hNCL (CIB1, NCL Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AT5G48740 O3_pECIA2

    Plasmid

    #114963

    Purpose

    Bait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G48740 (AT5G48740 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AT5G59650 O4_pECIA2

    Plasmid

    #114964

    Purpose

    Bait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G59650 (AT5G59650 Mustard Weed)
    

    Use

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein (Copper-Inducible)

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Fgr-SH2

    Plasmid

    #46427

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Fgr (FGR Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 137-245

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP SRBC

    Plasmid

    #68399

    Purpose

    Expression of fluorescently tagged SRBC in mammalian cells

    Depositor

    Rob Parton

    Article

    Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

    Insert

    SRBC (Cavin3 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCIBN-LacI

    Plasmid

    #103814

    Purpose

    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-LacI (CIB1, lacI E. coli, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_RBM45_WT

    Plasmid

    #82892

    Purpose

    Gateway Donor vector containing RBM45, part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, David Root, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    RBM45 (RBM45 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid

    #157693

    Purpose

    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pENTR1A-PIK3CA E545K

    Plasmid

    #180032

    Purpose

    Entry vector of PIK3CA E545K for gateway cloning.

    Depositor

    Xin Chen

    Article

    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv

    Insert

    PIK3CA E545K (PIK3CA Human)
    

    Use

    Entry vector

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    E545K

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge