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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,602 results for: REP

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Showing: 5421 - 5440 of 31602 results

  3. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Laure Crabbe
    Article
    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Available Since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122085-plasmid-map-sequence-id-238127

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  6. pCAG-FLAG-puro-PTPIP51(1-150_175-470)

    Plasmid
    #170537
    Purpose
    Expresses PTPIP51 FFAT-like motif (151-175) deletion mutant (1-150_176-470) in human HeLa cells after transfection
    Depositor
    Byung Il Lee
    Article
    Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:
    Insert
    Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    deleted amino acids 151-175
    Promoter
    CAG promoter
    Available Since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only

  7. pMXs-E55A-ATPIF1

    Plasmid
    #85404
    Purpose
    Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase
    Depositor
    David Sabatini
    Article
    Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1
    Insert
    ATPIF1 (ATP5IF1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    E55A point mutation abrogating the ability of ATP…
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. Epac-S-H27

    Plasmid
    #170336
    Purpose
    CFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.
    Insert
    CFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain
    Promoter
    CMV
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170336-plasmid-map-sequence-id-335892

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  11. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid
    #122056
    Purpose
    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P
    Depositor
    Stephen Tapscott
    Article
    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do
    Insert
    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    MyoD chimeric protein substituted with the NeuroD…
    Available Since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122056-plasmid-map-sequence-id-235399

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  15. pSems-leader-mEGFP-MBP-TMD(ALA7)

    Plasmid
    #193051
    Purpose
    Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    ALA7-TMD
    Tags
    Ig k-chain leader sequence, MBP, and mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    193051-plasmid-map-sequence-id-386362

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  16. pelB-MBP-P66-ss

    Plasmid
    #137045
    Purpose
    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66949.1 (p66 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature P66: lacks the P66 signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137045-plasmid-map-sequence-id-291951

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Showing: 5421 - 5440 of 31602 results