We narrowed to 19,002 results for: Tec

1. EK0425 CMV-TO-BFPmut-stop-Bdnf.t+54@202 (FLP-IN)

   Plasmid

   #191147

   Purpose

   Expression of the sequence matching the 54 extra bases in EK0362 in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   Bdfn.t+54
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)

   Plasmid

   #191150

   Purpose

   Expression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s1L153:EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)

   Plasmid

   #191152

   Purpose

   Expression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:Bdnf.s1L36:EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRB121

   Plasmid

   #181945

   Purpose

   aTc-inducible expression of dimerized mNeonGreen (mNG-mNG)

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   mNeonGreen-mNeonGreen; tetR (AX04_RS23485 Synthetic)
   

   Expression

   Bacterial

   Mutation

   See Depositor Comments Below

   Promoter

   mNG-mNG-PLtetO-1; tetR-J23106

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSNRW-Z3-Nluc

   Plasmid

   #174486

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z3-Nluc
   

   Tags

   6x Histidine tag

   Expression

   Bacterial

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2369-Tier1-PhCMV_KRAB

   Plasmid

   #169571

   Purpose

   Tier-1 vector encoding PhCMV-driven KRAB transsilencer domain (PhCMV-KRAB-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven Kruppel associated box domain
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIALD2E

   Plasmid

   #185866

   Purpose

   Knocking out ALD6

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD(-125, 40)- ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. rtTA-sg-CMV51

   Plasmid

   #188484

   Purpose

   gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   None

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCS-Clover4-LUMABS-DEN1

   Plasmid

   #183487

   Purpose

   To express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.

   Depositor

   Zongwen Jin, Jun Chu

   Article

   Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

   Insert

   Clover4-LUMABS-DEN1
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. L1-AA027

    Plasmid

    #185752

    Purpose

    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Insert

    HygR2
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS2341_Tier3(SB)-Puro

    Plasmid

    #169639

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pgTS40

    Plasmid

    #169630

    Purpose

    pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    U6-driven gRNA expression and PCAG-driven SpCas9 expression
    

    Expression

    Mammalian

    Promoter

    U6 / PCAG

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS1041

    Plasmid

    #169619

    Purpose

    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVH228-Tier1-OVanO2-PCMVmin2-SEAP

    Plasmid

    #169564

    Purpose

    Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    vanillic acid-controlled SEAP production
    

    Expression

    Mammalian

    Promoter

    VanO2-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH1001-Tier2(ColE1 ori)

    Plasmid

    #169599

    Purpose

    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS2351_Tier3(SB)-Zeo

    Plasmid

    #169648

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven ZeoR expression
    

    Expression

    Mammalian

    Promoter

    PRPBSA

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS1040

    Plasmid

    #169618

    Purpose

    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTS1039

    Plasmid

    #169617

    Purpose

    Tier-2 vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFOX11-Tier1-PhCMV-Clover

    Plasmid

    #169508

    Purpose

    Tier-1 vector encoding PhCMV-driven gren fluorescent protein clover (PhCMV-clover-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven green fluorescent protein clover
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTS2370-Tier1-PhCMV-TetR_F-type

    Plasmid

    #169573

    Purpose

    Tier-1 vector encoding PhCMV-driven tetracycline-dependent F-type-based transactiavtor (PhCMV-TetR-F-type-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled transactivator
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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